# US 7,745,699 B2 · Weed controller metabolism proteins, genes thereof and use of the same > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 7,745,699 B2 - Document ID: US7745699B2 - Status: Granted - Kind: B2 - Application number: 10/492,928 - Filed: 2002-10-17 - Published: 2005-04-21 - Granted: 2010-06-29 - Earliest claimed: 2001-10-19 - Assignees: Sumitomo Chemical Company, Limited - Inventors: Hiroki Nakajima; Fujio Mukumoto; Masanao Takaishi - Web page: https://patentgrep.com/patent/US7745699B2 ## CPC - C12N 15/8274: {for herbicide resistance} - A61K 38/16: Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof {(enzyme inhibitors A61K38/005)} - C07K 14/36: from Actinomyces; from Streptomyces (G) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) ## Abstract DNA encoding a herbicide metabolizing protein. Such DNA may, for example, be employed to produce herbicidally resistant plants. ## Claims ### Claim 1 An isolated DNA encoding a herbicide metabolizing protein, wherein said protein is selected from the group consisting of: (a) a protein comprising the amino acid sequence of SEQ ID NO: 224; (b) a protein having an ability to convert in the presence of an electron transport system containing an electron donor, a compound of formula (II) to a compound of formula (III), and comprising an amino acid sequence which has at least 90% sequence identity with the amino acid sequence of SEQ ID NO: 224, and which contains an amino acid sequence encoded by a DNA amplifiable by a polymerase chain reaction with a primer comprising the nucleotide sequence of SEQ ID NO: 124 and a primer comprising the nucleotide sequence of SEQ ID NO: 129, and which retains the cysteine at a position corresponding to amino acid number 347 in the amino acid sequence of SEQ ID NO: 224; and (c) a protein having an ability to convert in the presence of an electron transport system containing an electron donor, a compound of formula (II) to a compound of formula (III), and comprising an amino acid sequence encoded by a DNA amplifiable by a polymerase chain reaction with a primer comprising the nucleotide sequence of any one of SEQ ID NOs: 124 to 128, a primer comprising the nucleotide sequence of SEQ ID NO: 129 and as a template a chromosomal DNA of Streptomyces steffisburgensis. ### Claim 2 An isolated DNA comprising the nucleotide sequence of SEQ ID NO: 234. ### Claim 3 (depends on 1) The DNA according to claim 1, comprising a nucleotide sequence encoding an amino acid sequence of said protein, wherein the codon usage in said nucleotide sequence is within the range of plus or minus 4% of the codon usage in genes from the species of a host cell to which the DNA is introduced and the GC content of said nucleotide sequence is at least 40% and at most 60%. ### Claim 4 An isolated DNA comprising the nucleotide sequence shown in SEQ ID NO: 393. ### Claim 5 (depends on 1) An isolated DNA in which a DNA having a nucleotide sequence encoding an intracellular organelle transit signal sequence is linked upstream of the DNA according to claim 1 in frame. ### Claim 6 (depends on 1) An isolated DNA in which the DNA according to claim 1 and a promoter functional in a host cell are operably linked. ### Claim 7 (depends on 1) A vector comprising the DNA according to claim 1. ### Claim 8 (depends on 1) A method of producing a vector comprising a step of inserting the DNA according to claim 1 into a vector replicable in a host cell. ### Claim 9 (depends on 1) A transformant in which the DNA according to claim 1 is introduced into a host cell. ### Claim 10 (depends on 9) The transformant according to claim 9, wherein the host cell is a microorganism cell or a plant cell. ### Claim 11 (depends on 1) A method of producing a transformant comprising a step of introducing into a host cell, the DNA according to claim 1. ### Claim 12 (depends on 9) A method of producing a protein having the ability to convert a compound of formula (II) to a compound of formula (III), said method comprising a step of culturing the transformant according to claim 9 and recovering the produced said protein. ### Claim 13 (depends on 1) A method of giving a plant resistance to a herbicide, said method comprising step of introducing into and expressing in a plant cell, the DNA according to claim 1. ### Claim 14 (depends on 1) A method of detecting a DNA encoding a protein having the ability to convert a compound of formula (II) to a compound of formula (III), said method comprising a step of detecting a DNA to which a probe is hybridized in a hybridization using as the probe the DNA according to claim 1. ### Claim 15 (depends on 3) The DNA according to claim 3 comprising the nucleotide sequence of SEQ ID NO: 393.