# US 7,745,695 B2 · Generation of plants with altered oil content

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- Number: US 7,745,695 B2
- Document ID: US7745695B2
- Status: Granted
- Kind: B2
- Application number: 11/597,390
- Filed: 2005-05-26
- Published: 2009-06-25
- Granted: 2010-06-29
- Earliest claimed: 2004-05-28
- Assignees: Agrinomics LLC
- Inventors: John Davies; Sandra Peters; Hein Tsoeng Ng
- Web page: https://patentgrep.com/patent/US7745695B2

## CPC

- C12N 15/8247: {involving modified lipid metabolism, e.g. seed oil composition}
- C07K 14/415: from plants
- C12N 9/1205: {Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

The present invention is directed to plants that display an altered oil content phenotype due to altered expression of a HIO1005 nucleic acid. The invention is further directed to methods of generating plants with an altered oil content phenotype.

## Claims

### Claim 1

A method of generating a plant having a high oil phenotype comprising identifying a plant that has an allele in its HIO1005 gene that results in increased oil content compared to plants lacking the allele and generating progeny of said identified plant, wherein the generated progeny inherit the allele and have the high oil phenotype.

### Claim 2 (depends on 1)

The method of claim 1 that employs candidate gene/QTL methodology.

### Claim 3 (depends on 1)

The method of claim 1 that employs TILLING methodology.
