# US 7,745,671 B2 · Method for producing scyllo-inositol

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- Number: US 7,745,671 B2
- Document ID: US7745671B2
- Status: Granted
- Kind: B2
- Application number: 10/576,030
- Filed: 2004-10-14
- Published: 2006-10-26
- Granted: 2010-06-29
- Earliest claimed: 2003-10-14
- Assignees: Hokko Chemical Industry Co., Ltd.
- Inventors: Masanori Yamaguchi; Yuichi Kita; Tetsuya Mori; Kenji Kanbe; Akihiro Tomoda; Atsushi Takahashi; Wakako Ichikawa
- Web page: https://patentgrep.com/patent/US7745671B2

## CPC

- C12P 7/02: containing a hydroxy group
- C07C 29/145: with hydrogen or hydrogen-containing gases
- C12N 9/0004: {Oxidoreductases (1.)}
- C12N 9/0006: {acting on CH-OH groups as donors (1.1)}
- C12P 7/18: polyhydric
- C12P 7/26: Ketones
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C07C: ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

It is intended to provide a novel NAD+-independent myo-inositol 2-dehydrogenase which converts myo-inositol into scyllo-inosose in the absence of NAD+; a novel enzyme scyllo-inositol dehydrogenase which stereospecifically reduces scyllo-inosose into scyllo-inositol in the presence of NADH or NADPH; and a novel microorganism which belongs to the genus Acetobacter or Burkholderia and can convert myo-inositol into scyllo-inositol. By using these enzymes or the microorganism, scyllo-inositol is produced. Furthermore, scyllo-inositol is purified by adding boric acid and a metal salt to a liquid mixture containing scyllo-inositol and a neutral saccharide other than scyllo-inositol to form a scyllo-inositol/boric acid complex, separating the complex from the liquid mixture, dissolving the thus separated complex in an acid to give an acidic solution or an acidic suspension and then purifying scyllo-inositol from the acidic solution or the acidic suspension.

## Claims

### Claim 1

A method for producing a purified scyllo-inositol, comprising:
a first step of precipitating a scyllo-inositol/boric acid complex by adding boric acid and a metal salt into a liquid mixture containing scyllo-inositol and neutral sugar other than scyllo-inositol in an amount by mol two times or more that of scyllo-inositol dissolved in the liquid mixture, and by adjusting the pH of the liquid mixture to 8.0 to 11.0;
a second step of separating the complex from the liquid mixture;
a third step of dissolving the separated complex into acid to cleave into scyllo-inositol and boric acid to obtain an acidic solution or acidic suspension; and
a fourth step of adding ethanol or methanol to the acidic solution or acidic suspension to precipitate scyllo-inositol and purifying the scyllo-inositol from the acidic solution or acidic suspension,
wherein the metal salt to be added is one or more kinds of metal salts selected from the group consisting of NaCl, NaHCO3, Na2CO3, Na2SO4, NaHSO4, NaH2PO4, Na2HPO4, Na3PO4, borax, KCl, KHCO3, K2CO3, K2SO4, KHSO4, KH2PO4, K2HPO4, K3PO4, MgCl2, MgCO3, and MgSO4, and wherein the ethanol is added in a volume 0.3 to 3 times the volume of the acidic solution or acidic suspension, or the methanol is added in a volume 0.3 to 5 times the volume of the acidic solution or acidic suspension.

### Claim 2 (depends on 1)

The method according to claim 1, wherein, in the first step, the amounts of the boric acid and metal salt to be added are two to three times the amount by mol of the scyllo-inositol dissolved in the liquid mixture.

### Claim 3 (depends on 1)

The method according to claim 1, wherein, in the first step, the pH of the liquid mixture is adjusted to 9.0 to 10.0.

### Claim 4 (depends on 1)

The method according to claim 1, wherein the ethanol is added in a volume 0.6 to 1.5 times the volume of the acidic solution or acidic suspension, or the methanol is added in a volume 0.9 to 2 times the volume of the acidic solution or the acidic suspension.