# US 7,745,644 B2 · Antibiotic compounds

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 7,745,644 B2
- Document ID: US7745644B2
- Status: Granted
- Kind: B2
- Application number: 10/560,665
- Filed: 2004-07-20
- Published: 2008-12-11
- Granted: 2010-06-29
- Earliest claimed: 2003-07-24
- Assignees: Merck Sharp & Dohme Corp.; Merck Sharp and Dohme de Espana, S.A.
- Inventors: Angela Basilio; Olga Genilloud; Pilar Hernandez; Sheo Bux Singh; Jose Rueben Tormo; Jun Wang
- Web page: https://patentgrep.com/patent/US7745644B2

## CPC

- C12N 1/205: Bacterial isolates
- A61P 31/04: Antibacterial agents
- C07D 493/08: Bridged systems
- C12P 17/06: containing a six-membered hetero ring, e.g. fluorescein
- C12R 2001/465: Streptomyces
- Y02A 50/30: Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C07D: HETEROCYCLIC COMPOUNDS (macromolecular compounds C08)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

Fermentation of a nutrient medium with a eubacterium Streptomyces sp. yields a novel antibacterial compound of structure (I).

## Claims

### Claim 1

An isolated compound of structural formula (I):
or a pharmaceutically acceptable salt thereof.

### Claim 2

A process for the preparation of an isolated compound of structural formula (I):
which comprises the cultivation of a Streptomyces sp. with ATCC# PTA-5316 (MA7327) or ATCC#PTA-5317 (MA7331) or a natural mutant thereof in a nutrient medium and isolating the Compound (I) from the fermentation broth.

### Claim 3 (depends on 2)

The process of claim 2 wherein the fermentation is conducted at a temperature of about 10° C. to about 40° C.

### Claim 4 (depends on 3)

The process of claim 3, wherein the fermentation is conducted at a temperature of about 28° C.

### Claim 5 (depends on 1)

A pharmaceutical composition comprising a pharmaceutically acceptable carrier and an effective amount of a compound of structure (I) according to claim 1.