# US 7,745,598 B2 · CpG single strand deoxynucleotides for use as adjuvant

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 7,745,598 B2
- Document ID: US7745598B2
- Status: Granted
- Kind: B2
- Application number: 12/329,398
- Filed: 2008-12-05
- Published: 2009-06-18
- Granted: 2010-06-29
- Earliest claimed: 2004-11-29
- Assignees: Changchun Huapu Biotechnology Co., Ltd.
- Inventors: Liying Wang; Musheng Bao; Yongli Yu
- Web page: https://patentgrep.com/patent/US7745598B2

## CPC

- A61K 39/39: characterised by the immunostimulating additives, e.g. chemical adjuvants
- A61P 1/16: for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
- A61P 31/12: Antivirals
- A61P 31/18: for HIV
- A61P 31/20: for DNA viruses
- A61P 37/02: Immunomodulators
- A61P 43/00: Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
- C12N 15/117: Nucleic acids having immunomodulatory properties, e.g. containing CpG-motifs
- A61K 2039/55561: {CpG containing adjuvants; Oligonucleotide containing adjuvants}
- C12N 2310/16: Aptamers
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention provides an adjuvant, which includes at least one single strand deoxynucleotide containing a CpG dinucleotide. The single strand deoxynucleotide comprises one or more CpG dinucleotides. When used in combination with rabies vaccine, HBV vaccine or other vaccines, the adjuvant can significantly improve the immune effect of the vaccine.

## Claims

### Claim 1

A method of improving immunogenicity of a vaccine, comprising coadministering said vaccine to a subject with a single strand deoxynucleotide, comprising the nucleotide sequence of SEQ ID NO: 167 and said vaccine.

### Claim 2 (depends on 1)

The method of claim 1, wherein bases of the deoxynucleotide are modified by one or more modifications selected from the group consisting of non-sulfur modification, sulfur modification, partial sulfur modification, rare base modification, methylation modification, and other modifications where sulfhydryl, Aminolinker C6 and Thiol-C6 S-S are used to couple to other substances.

### Claim 3 (depends on 1)

The method of claim 1, wherein the single strand deoxynucleotide is used alone or in combination with a non-nucleic acid adjuvant including aluminum adjuvant, Freund's adjuvant, MPL, or emulsion.

### Claim 4 (depends on 1)

The method of claim 1, wherein the single strand deoxynucleotide is admixed with or chemically coupled to a vaccine, or the single strand deoxynucleotide is cloned into a DNA vaccine.

### Claim 5 (depends on 1)

The method of claim 1, wherein the vaccine is selected from the group consisting of hepatitis B virus blood-derived vaccine, hepatitis B virus genetic engineering protein vaccines, HBV virus vector vaccine, hepatitis B virus bacterium vector vaccine, hepatitis B virus transgenic plant vaccine, rabies virus blood-derived vaccine, rabies virus genetic engineering protein vaccines, rabies virus vector vaccine, rabies virus bacterium vector vaccine, and rabies virus transgenic plant vaccine, and the DNA vaccine is selected from the group consisting of hepatitis B virus DNA vaccine and rabies DNA vaccine.

### Claim 6 (depends on 1)

The method of claim 1, further comprising administering non-nucleic acid adjuvants.

### Claim 7 (depends on 1)

The method of claim 1, wherein the subject is human.

### Claim 8 (depends on 1)

The method of claim 1, wherein the subject is a mammal.