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US 7,745,594 B2Granted
Nucleic acid-based logic circuits
AssigneeCalifornia Institute of Technology
InventorsGeorg Seelig, David Soloveichik, Erik Winfree, David Zhang
Life of this patent
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Abstract
This invention relates to nucleic acid-based logic gates. The invention further relates to circuits comprising nucleic acid-based logic gates and methods of performing operations with the gates and circuits provided herein.
Claims37
A logic gate complex comprising:
- a) at least one output oligonucleotide comprising:
- i) a first region comprising at least one toehold binding domain that forms a duplex with a complementary nucleic acid sequence of a gate oligonucleotide;
- ii) a second region linked with the first region and comprising at least one single-stranded domain comprising an oligonucleotide that, upon release, functions as an input oligonucleotide sequence for a subsequent logic gate or as a signal sequence for detection of an operation, or a combination thereof;
- iii) a third region linked with the second region and comprising a nucleic acid sequence that forms a duplex with a complementary nucleic acid sequence of a gate oligonucleotide;
- b) at least one gate oligonucleotide comprising:
- i) a first region comprising a single-stranded toehold binding domain complementary to an input oligonucleotide sequence;
- ii) a second region comprising a nucleic acid sequence complementary to, and forming a duplex with, the first and third regions of the output oligonucleotide.
4 more elements
Dependencies are inferred from the claim text.