# US 7,745,589 B1 · Antibodies and unnatural substrates of prenylation enzymes for use in detecting and isolating prenylated proteins

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- Number: US 7,745,589 B1
- Document ID: US7745589B1
- Status: Granted
- Kind: B1
- Application number: 11/459,299
- Filed: 2006-07-21
- Granted: 2010-06-29
- Earliest claimed: 2005-07-21
- Assignees: University of Kentucky Research Foundation
- Inventors: Hans Peter Spielmann; Douglas A. Andres
- Web page: https://patentgrep.com/patent/US7745589B1

## CPC

- C07K 16/44: against material not provided for elsewhere {, e.g. haptens, metals, DNA, RNA, amino acids}
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

Unnatural substrates of prenylation enzymens and antibodies that recognize unique moieties of prenylated proteins, which unique moieties are transferred from the unnatural substrates are used for detecting and isolating prenylated proteins, and for screening for inhibitors of prenylation enzymes.

## Claims

### Claim 1

An isolated antibody that recognizes a unique moiety of a protein modified therewith, the unique moiety being selected from the group consisting of: para-nitro-anilinogeranyl moiety, ortho-fluoro-anilinogeranyl moiety, para-cyano-anilinogeranyl moiety, para-nitro-anilinofarnesyl moiety, meta-cyano-anilinogeranyl moiety, and para-bromo-anilinogeranyl moiety, wherein the antibody that recognizes the unique moiety of the modified protein does not recognize farnesylated proteins or geranylgeranylated proteins.

### Claim 2

An isolated antibody that recognizes a unique moiety of a protein modified therewith, the unique moiety being selected from the group consisting of: anilinogeranyl moiety, para-fluoro-anilinogeraniol moiety, meta-fluoro-anilinogeranyl moiety, para-methyl-anilinogeranyl moiety, meta-methyl-anilinogeranyl moiety, and meta-bromo-anilinogeranyl moiety, wherein the antibody that recognizes the unique moiety of the modified protein does not recognize farnesylated proteins or geranylgeranylated proteins.

### Claim 3 (depends on 2)

The isolated antibody according to claim 2, wherein the unique moiety is an anilinogeranyl moiety.

### Claim 4 (depends on 3)

The isolated antibody according to claim 3, wherein the the unique moiety is an anilinogeranyl moiety of anilinogeranyl diphosphate, or an anilinogeranyl moiety of anilinogeranyl alcohol.

### Claim 5

An isolated monoclonal antibody that recognizes an anilinofarnesyl moiety of a protein modified therewith, wherein the antibody that recognizes the unique moiety of the modified protein does not recognize farnesylated proteins or geranylgeranylated proteins.

### Claim 6 (depends on 1)

The isolated antibody according to claim 1, wherein the unique moiety is a pNO2-anilinogeranyl moiety.

### Claim 7 (depends on 6)

The isolated antibody according to claim 6, wherein the unique moiety is a pNO2-anilinogeranyl moiety of pNO2-8-anilinogeranyl diphosphate, or a pNO2-anilinogeranyl moiety of pNO2-8-anilinogeranyl alcohol.

### Claim 8 (depends on 1)

The isolated antibody according to claim 1, wherein the unique moiety is a para-cyano-anilinogeranyl moiety or para-notro-anilinofarnesyl moiety.

### Claim 9 (depends on 1)

The isolated antibody according to claim 1, wherein the antibody is an isolated monoclonal antibody, wherein the unique moiety is a para-nitro-anilinogeranyl moiety.

### Claim 10 (depends on 9)

The isolated monoclonal antibody according to claim 9, wherein the antibody recognizes an anilinogeranyl moiety of a protein modified with the anilinogeranyl moiety.