# US 7,745,584 B2 · Antibodies to sulfated carbohydrates

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- Number: US 7,745,584 B2
- Document ID: US7745584B2
- Status: Granted
- Kind: B2
- Application number: 11/751,863
- Filed: 2007-05-22
- Published: 2008-01-10
- Granted: 2010-06-29
- Earliest claimed: 2006-05-22
- Assignees: California Institute of Technology
- Inventors: Sarah E. Tully; Linda Hsieh-Wilson
- Web page: https://patentgrep.com/patent/US7745584B2

## CPC

- C07K 16/18: against material from animals or humans
- A61K 2039/505: {comprising antibodies}
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

The present invention relates to antibodies and antigen-binding portions thereof that specifically bind to chondroitin sulfate, particularly CS-A, CS-C and CS-E tetrasaccharides. The present invention also relates to methods of making anti-CS antibodies, pharmaceutical compositions comprising these antibodies and methods of using the antibodies and compositions thereof for diagnosis and treatment.

## Claims

### Claim 1

An isolated antibody comprising a heavy chain and a light chain, wherein said heavy chain comprises the three heavy chain complementarity determining regions (CDRs) of antibody 2D11-2A10 that is produced by a cell line deposited under ATCC accession number PTA10049, and wherein said light chain comprises the three light chain CDRs of said antibody 2D11-2A10, wherein the isolated antibody binds to a mammalian chondroitin sulfate E oligosaccharide comprising one or more disaccharides in the following structure:
wherein n is greater than 0.

### Claim 2 (depends on 1)

The isolated antibody of claim 1, wherein the isolated antibody is an isolated humanized antibody.

### Claim 3 (depends on 1)

The isolated antibody of claim 1, wherein the antibody is monoclonal.

### Claim 4 (depends on 1)

The isolated antibody of claim 1, wherein the antibody selectively binds to chondroitin sulfate E (CS-E) as measured with a chondroitin sulfate microarray that comprises CS-E immobilized thereon.

### Claim 5 (depends on 1)

The isolated antibody of claim 1, wherein the disaccharide is of the structure:
wherein n equals 1.

### Claim 6 (depends on 1)

The isolated antibody of claim 1, wherein n equals 2.

### Claim 7 (depends on 1)

A cell line that produces the antibody of claim 1.

### Claim 8 (depends on 1)

The isolated antibody of claim 1, wherein the oligosaccharide is a tetrasaccharide or a disaccharide.

### Claim 9 (depends on 1)

The isolated antibody of claim 1, wherein the isolated antibody is 2D11-2A10 produced by a cell line deposited under ATCC accession number PTA10049.