# US 7,745,558 B2 · Entrapped β-cyclodextrin polymer and a method of preparing the same

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 7,745,558 B2
- Document ID: US7745558B2
- Status: Granted
- Kind: B2
- Application number: 11/947,035
- Filed: 2007-11-29
- Published: 2008-08-14
- Granted: 2010-06-29
- Earliest claimed: 2006-11-29
- Assignees: Korea Institute of Science and Technology
- Inventors: Man Ho Choi; Dong Hyun Kim; Bong Chul Chung; Ju-Yeon Moon
- Web page: https://patentgrep.com/patent/US7745558B2

## CPC

- C08F 251/00: Macromolecular compounds obtained by polymerising monomers on to polysaccharides or derivatives thereof
- C08B 37/0012: {Cyclodextrin [CD], e.g. cycle with 6 units (alpha), with 7 units (beta) and with 8 units (gamma), large-ring cyclodextrin or cycloamylose with 9 units or more; Derivatives thereof}
- C08L 5/16: Cyclodextrin; Derivatives thereof
- C08L 51/02: grafted on to polysaccharides
- G01N 33/548: Carbohydrates, e.g. dextran
- G01N 33/743: {Steroid hormones}
- G01N 2600/00: Assays involving molecular imprinted polymers/polymers created around a molecular template
- C08F: MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08B: POLYSACCHARIDES; DERIVATIVES THEREOF (polysaccharides containing less than six saccharide radicals attached to each other by glycosidic linkages C07H; fermentation or enzyme-using processes C12P19/00; sugar industry C13; production of cellulose D21)
- C08L: COMPOSITIONS OF MACROMOLECULAR COMPOUNDS (compositions based on polymerisable monomers C08F, C08G; artificial filaments or fibres D01F; textile treating compositions D06)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

Provided are entrapped β-cyclodextrin polymers and a method of preparing the same. Particularly, entrapped β-cyclodextrin polymers are prepared by adding epichlorohydrin to β-cyclodextrin to prepare a polymer in a gel state, entrapping the polymer by entrapment of metal ions on the surface of the polymer and pulverizing the entrapped polymer to prepare powders. According to the entrapped β-cyclodextrin polymers, steroid hormones contained in a biological sample can be simply extracted without using an additional device required in conventional solid-phase extraction.

## Claims

### Claim 1

A method of preparing entrapped β-cyclodextrin polymers, the method comprising:
preparing β-cyclodextrin polymers in a gel state by dissolving β-cyclodextrin in an aqueous alkali solution and adding epichlorohydrin to the solution;
preparing entrapped β-cyclodextrin polymers by immersing the β-cyclodextrin polymers in a gel state in a metal salt solution followed by entrapping on the surface of the β-cyclodextrin polymers; and
washing, drying and pulverizing the entrapped polymers to powderize the entrapped β-cyclodextrin polymers
wherein the metal salt is selected from the group consisting of CaCl2, NaCl, Ca(NO3)2 and CaSO4.

### Claim 2 (depends on 1)

The method of claim 1, wherein the alkali aqueous solution is prepared using an alkali metal hydroxide.

### Claim 3 (depends on 2)

The method of claim 2, wherein the alkali metal hydroxide is NaOH or KOH.

### Claim 4 (depends on 1)

The method of claim 1, wherein the concentration of the metal salt solution is in the range of 0.2 to 0.3 M.