# US 7,745,399 B2 · Hsp70 peptide stimulating natural killer (NK) cell activity and uses thereof

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 7,745,399 B2
- Document ID: US7745399B2
- Status: Granted
- Kind: B2
- Application number: 12/395,818
- Filed: 2009-03-02
- Published: 2009-09-24
- Granted: 2010-06-29
- Earliest claimed: 2000-09-13
- Assignees: Multimmune GmbH
- Inventors: Gabriele Multhoff
- Web page: https://patentgrep.com/patent/US7745399B2

## CPC

- C07K 14/47: from mammals
- A61K 38/1709: {from mammals}
- A61P 31/12: Antivirals
- C07K 16/18: against material from animals or humans
- C12N 5/0646: {Natural killers cells [NK], NKT cells}
- A61K 2035/124: {the cells being hematopoietic, bone marrow derived or blood cells}
- C07K 2317/34: Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
- C12N 2501/07: Heat shock proteins
- C12N 2501/2302: Interleukin-2 (IL-2)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention relates to an immunostimulatory peptide derived from an Hsp70 protein and peptides comprising said immunostimulatory peptide. Furthermore the present invention pertains to polynucleotides encoding said peptide, vectors comprising said polynucleotides, fusion (poly)peptides comprising said peptide and compositions comprising said peptide. In addition the present invention relates to the use of said peptide, polynucleotide, vector or fusion (poly)peptide, for the preparation of pharmaceutical compositions for the treatment of diseases and for the stimulation of natural killer cell (NK cell) activity.

## Claims

### Claim 1

A method for treating a disorder treatable by stimulating NK cell proliferation or NK cell cytolytic activity comprising administering to a host having said disorder a composition comprising a peptide, said peptide being at least 13 and no more than 30 amino acids in length comprising an amino acid sequence selected from the group consisting of a) TKDNNLLGRFELSG corresponding to amino acid residues 450 to 463 of SEQ ID NO:8 wherein Ser can also be Thr and b) an amino acid sequence which deviates from the amino acid sequence of (a) by way of amino acid substitution, insertion, or deletion wherein amino acids TKDN corresponding to positions 450 to 453 of SEQ ID NO:8 and the amino acid Ser which can also be Thr are retained, wherein said peptide stimulates natural killer cell activity.

### Claim 2 (depends on 1)

The method of claim 1, wherein said peptide comprises the amino acid sequence EGERAMTKDNNLLGRFELXG (SEQ ID NO: 3) wherein X is T or S or a fragment or derivative thereof.

### Claim 3 (depends on 1)

The method of claim 1 wherein said disorder is a carcinoma, wherein host cells express Hsp70.