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US 7,745,207 B2Granted
Microfluidic devices
AssigneeIntegenX Inc.
InventorsStevan B. Jovanovich, Iuliu I. Blaga
Life of this patent
Estimating the term…
Abstract
Methods and devices for the interfacing of microchips to various types of modules are disclosed. The technology disclosed can be used as sample preparation and analysis systems for various applications, such as DNA sequencing and genotyping, proteomics, pathogen detection, diagnostics and biodefense.
Claims18
A method of mixing solutions comprising:
- a) providing a microfluidic device comprising:
- (i) a first microfluidic channel comprising a first pump and a first solution;
- (ii) a second microfluidic channel comprising a second, opposing pump and a second solution;
- and
- (iii) a third microfluidic channel,
- wherein each pump comprises an inlet valve, a pump valve and an outlet valve, and the first, second and third channels intersect at a common junction that is not a valve;
- and
- b) performing a pumping cycle comprising:
- (i) opening the inlet valves and closing the outlet valves;
- (ii) opening the pump valves, wherein the first and second solutions are pumped into the pump valves;
- (iii) closing the inlet valves and opening the outlet valves;
- and
- (iv) closing the pump valves, wherein the solutions in the pump valves are pumped into the third channel in laminar flow and wherein opening the outlet valves and closing the pump valves results in back and forth movement of the solutions, wherein the solutions are mixed.
10 more elements
A method of mixing solutions comprising:
- a) providing a microfluidic device comprising a router comprising:
- (i) a first microfluidic channel comprising a first valve and a first port comprising a first solution;
- (ii) a second microfluidic channel comprising a second valve and a second port comprising a gas;
- (iii) a third microfluidic channel comprising a third valve and a third port comprising a second solution;
- (iv) a fourth microfluidic channel comprising a fourth valve;
- and
- (v) a reaction chamber
- wherein the first, second, third and fourth channels connect to a central diaphragm valve and the fourth channel connects with the reaction chamber;
- b) pumping the first and second solutions from the first and third ports and air from the second port into the reaction chamber to form boluses separated by air, wherein mixing of the solutions is aided by the back and forth movement of the two solutions into the second channel during multiple pumping steps.
6 more elements
Dependencies are inferred from the claim text.