# US 7,745,199 B2 · Bacterial consortium NBC2000 and method for biologically treating endocrine disrupters using the NBC2000

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- Number: US 7,745,199 B2
- Document ID: US7745199B2
- Status: Granted
- Kind: B2
- Application number: 11/587,835
- Filed: 2005-04-28
- Published: 2008-10-09
- Granted: 2010-06-29
- Earliest claimed: 2004-04-28
- Inventors: Sung-gie Lee
- Web page: https://patentgrep.com/patent/US7745199B2

## CPC

- B09C 1/10: microbiologically, {biologically} or by using enzymes
- C02F 3/341: {Consortia of bacteria}
- C10G 32/00: Refining of hydrocarbon oils by electric or magnetic means, by irradiation, or by using microorganisms
- C12N 1/205: Bacterial isolates
- C12P 1/04: by using bacteria
- C12P 39/00: Processes involving microorganisms of different genera in the same process, simultaneously
- C02F 2101/305: {Endocrine disruptive agents}
- C02F 2101/363: {PCB's; PCP's}
- C02F 2101/366: {Dioxine; Furan}
- C12R 2001/01: Bacteria or Actinomycetales {; using bacteria or Actinomycetales}
- C12R 2001/07: Bacillus
- C12R 2001/085: Bacillus cereus
- C12R 2001/38: Pseudomonas
- C12R 2001/385: Pseudomonas aeruginosa
- C12R 2001/425: Serratia
- B09C: RECLAMATION OF CONTAMINATED SOIL (gatherers for removing stone or the like from the soil A01B43/00; sterilising soil by steam A01G11/00; removing undesirable matter, e.g. rubbish, from the land E01H15/00)
- C02F: TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE (separation in general B01D; special arrangements on waterborne vessels of installations for treating water, waste water or sewage, e.g. for producing fresh water, B63J; adding materials to water to prevent corrosion C23F; treating radioactively-contaminated liquids G21F9/04; regeneration of reactants for recirculation into processes, see the relevant places for the processes)
- C10G: CRACKING HYDROCARBON OILS; PRODUCTION OF LIQUID HYDROCARBON MIXTURES, e.g. BY DESTRUCTIVE HYDROGENATION, OLIGOMERISATION, POLYMERISATION (cracking to hydrogen or synthesis gas C01B; cracking or pyrolysis of hydrocarbon gases to individual hydrocarbons or mixtures thereof of definite or specified constitution C07C; cracking to cokes C10B); RECOVERY OF HYDROCARBON OILS FROM OIL-SHALE, OIL-SAND, OR GASES; REFINING MIXTURES MAINLY CONSISTING OF HYDROCARBONS; REFORMING OF NAPHTHA; MINERAL WAXES
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

Disclosed is a novel bacterial consortium comprise bacterial strains useful for effectively treating endocrine disrupters and a method for treating endocrine disrupters using the same. The invention provides a method for biologically restoring soils, wastes and water, etc. which are polluted with chlorinated compounds such as polychlorinated biphenyl (PCBs), dioxin, pentachlorophenol (PCP), perchloroethylene (PCE), trichloroethylene (TCE) and 1,1,1-trichloroethane (1,1,1-TCA), etc., polycyclic aromatic hydrocarbons (PAH) and petroleum-tar acids, and toluene which are all known as representative endocrine disrupters.

## Claims

### Claim 1

A biologically pure culture of bacterial consortium NBC 2000, identified by Accession No. KCTC 10623BP, comprising Pseudomonas sp. Cy100 strain, Serratia sp. Aeng18 strain, Pseudomonas sp. Djhc strain, Pseudomonas sp. Ntar3 strain, Serratia sp. Ntar2 strain, Pseudomonas sp. EBC107 strain, Pseudomonas aeruginosa Tnh strain, Aeromonas hydrophila Aeng17 strain, Pseudomonas aeruginosa Pcpts strain, Stenotrophomonas maltophilia Ntar1 strain, Pseudomonas aeruginosa Sp300 strain, Chryseomonas luteola Gc501 strain, Chryseomonas sp. Gc500 strain, Chryseomonas luteola Gc300 strain, Brevundimonas vesicularis Cy101 strain, Brevundimonas vesicularis Cy102 strain, Brevundimonas vesicularis Cy103 strain, Bacillus stearothermophilus Bs100 strain, Bacillus stearothermophilus Cy104 strain, Bacillus sp. Cy105 strain, Bacillus sp. Cy106 strain, Bacillus sp. Cy107 strain, Bacillus cereus EBC106 strain, Bs101 strain which is petroleum-tar acid decomposition gram-positive bacteria, Nz2001 strain which is sulfur strain and W24 strain which is oil decomposition gram-negative bacteria.