# US 7,745,190 B2 · Endoribonuclease

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 7,745,190 B2
- Document ID: US7745190B2
- Status: Granted
- Kind: B2
- Application number: 12/067,769
- Filed: 2006-09-08
- Published: 2009-02-19
- Granted: 2010-06-29
- Earliest claimed: 2005-09-21
- Assignees: Takara Bio Inc.
- Inventors: Masamitsu Shimada; Masanori Takayama; Kiyoza Asada; Ikunoshin Kato
- Web page: https://patentgrep.com/patent/US7745190B2

## CPC

- C12N 9/22: Ribonucleases {[RNase]; Deoxyribonucleases [DNase]}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

A novel endoribonuclease activity exhibiting polypeptide; a nucleic acid coding for the polypeptide; a recombinant DNA comprising the nucleic acid; a transformant obtained by transformation using the recombinant DNA; a process for producing the polypeptide, characterized by including the steps of culturing the transformant and collecting the polypeptide from the culture; a process for producing single-strand RNA fragments, characterized by including the step of causing the polypeptide to act on a single-strand RNA; and a method of fragmenting a single-strand RNA.

## Claims

### Claim 1

A method for degrading a single-stranded RNA, the method comprising:
allowing a polypeptide having an endoribonuclease activity specific for single-stranded RNA which cleaves RNA in a nucleotide sequence-specific manner to act on a single-stranded RNA,
wherein said polypeptide has the amino acid sequence of SEQ ID NO: 1 or an amino acid sequence having 90% or more homology to the polypeptide of SEQ ID NO: 1.