# US 7,745,187 B2 · Sulfotransferase sequence variants

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- Number: US 7,745,187 B2
- Document ID: US7745187B2
- Status: Granted
- Kind: B2
- Application number: 12/398,700
- Filed: 2009-03-05
- Published: 2009-09-03
- Granted: 2010-06-29
- Earliest claimed: 2001-02-23
- Assignees: Mayo Foundation for Medical Education and Research
- Inventors: Robert R Freimuth; Eric D Wieben; Richard M Weinshilboum
- Web page: https://patentgrep.com/patent/US7745187B2

## CPC

- C12N 9/13: {transferring sulfur containing groups (2.8)}
- C07H 21/04: with deoxyribosyl as saccharide radical
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07H: SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS (derivatives of aldonic or saccharic acids C07C, C07D; aldonic acids, saccharic acids C07C59/105, C07C59/285; cyanohydrins C07C255/16; glycals C07D; compounds of unknown constitution C07G; polysaccharides, derivatives thereof C08B; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; sugar industry C13)

## Abstract

Isolated sulfotransferase nucleic acid molecules that include a nucleotide sequence variant and nucleotides flanking the sequence variant are described, as well as sulfotransferase allozymes. Methods for determining if a mammal is predisposed to thyroid disease or cancer also are described.

## Claims

### Claim 1

An isolated SULT1C1 polypeptide variant comprising the sequence set forth in SEQ ID NO: 2 wherein amino acid residue 60, 73, 111 or 255 of SEQ ID NO: 2 is substituted with a different amino acid.

### Claim 2 (depends on 1)

The isolated polypeptide of claim 1, wherein said amino acid sequence variant is an alanine at residue 60 of SEQ ID NO:2.

### Claim 3 (depends on 1)

The isolated polypeptide of claim 1, wherein said amino acid sequence variant is a glutamine at residue 73 of SEQ ID NO:2.

### Claim 4 (depends on 1)

The isolated polypeptide of claim 1, wherein said amino acid sequence variant is a phenylalanine at residue 111 of SEQ ID NO:2.

### Claim 5 (depends on 1)

The isolated polypeptide of claim 1, wherein said amino acid sequence variant is an alanine at residue 255 of SEQ ID NO:2.

### Claim 6 (depends on 1)

The isolated polypeptide variant of claim 1, wherein said polypeptide variant comprises amino acid substitutions at residues 60 and 255 of SEQ ID NO: 2 or at residues 73 and 255 of SEQ ID NO: 2.

### Claim 7 (depends on 1)

The isolated polypeptide of claim 1, wherein activity of said polypeptide is altered relative to a wild type SULT1C1 polypeptide.