# US 7,745,152 B2 · Method for determining genotoxicity

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- Number: US 7,745,152 B2
- Document ID: US7745152B2
- Status: Granted
- Kind: B2
- Application number: 11/286,216
- Filed: 2005-11-23
- Published: 2006-05-25
- Granted: 2010-06-29
- Earliest claimed: 2004-11-24
- Assignees: Roche Palo Alto LLC
- Inventors: John David Allard; Dee Aud; Guochun Liao; Gary Allen Peltz
- Web page: https://patentgrep.com/patent/US7745152B2

## CPC

- C12Q 1/6897: involving reporter genes operably linked to promoters
- C07K 14/705: Receptors; Cell surface antigens; Cell surface determinants {(tumour specific antigens C07K14/4748)}
- C12Q 1/6876: Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- A01K 2217/05: Animals comprising random inserted nucleic acids (transgenic)
- A01K 2217/075: inducing loss of function, i.e. knock out
- C12Q 2600/158: Expression markers
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A01K: ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS

## Abstract

Methods and reagents for determining the genotoxicity of a compound based on genomic responses to contact therewith are provided.

## Claims

### Claim 1

A method for determining the genotoxicity of a test compound, said
method comprising:
(a) contacting a viable murine myoblast test cell with said test compound;
(b) determining the change in expression level of a murine indicator gene selected from the group consisting of 4833427G06 Rik (RIKEN cDNA) (SEQ ID NO:5) and murine Dda3 (Differential display and activated by p53)
wherein an increase in expression of at least 1.5-fold in said murine myoblast test cell, compared to a murine myoblast cell comprising 4833427G06 Rik (RIKEN cDNA) (SEQ ID NO: 5) or murine Dda3, that is not exposed to the test compound, indicates that said test compound exhibits genotoxicity.

### Claim 2 (depends on 1)

The method of claim 1, wherein determining the change in expression level comprises measuring the amount of mRNA produced using RT-PCR.

### Claim 3 (depends on 1)

The method of claim 1, wherein determining the change in expression level comprises measuring the increase in signal produced by increased expression of a label.