# US 7,745,149 B2 · Tumor markers for ovarian cancer diagnosis

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- Number: US 7,745,149 B2
- Document ID: US7745149B2
- Status: Granted
- Kind: B2
- Application number: 12/326,369
- Filed: 2008-12-02
- Published: 2009-04-16
- Granted: 2010-06-29
- Earliest claimed: 2006-09-14
- Assignees: National Taiwan University
- Inventors: Song-Nan Chow
- Web page: https://patentgrep.com/patent/US7745149B2

## CPC

- G01N 33/57545: of the ovaries
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

The present invention relates to a tumor marker for diagnosis of ovarian cancer, which is selected from the group consisting of galectin-1, cathepsin B, MHC class I antigen, heat shock protein (HSP) 27, ubiquitin carboxy-termal esterase L1, plasma retinol-binding protein (PRBP), transthyretin, SH3 binding glutamate-rich protein, tubulin-specific chaperone A, RNA binding protein regulatory subunit, γ-actin, tropomyosin and calcium/calmodulin-stimulated cyclic nucleotide phosphatase. The ovarian cancer is diagnosed effectively and efficiently based on detecting the expression levels of the tumor markers in the invention from the ovarian tissue sample of an individual to be diagnosed.

## Claims

### Claim 1

A method for detecting ovarian cancer, which comprises the steps of:
(1) obtaining an ovarian tissue sample from an individual to be diagnosed and an ovarian tissue sample from a non-cancerous ovarian tissue;
(2) determining expression levels of thirteen tumor markers having the amino acid sequences of SEQ ID NOs: 1-13 in the ovarian tissue sample of the individual to be diagnosed and the non-cancerous ovarian tissue sample;
(3) comparing the expression level of each tumor marker in the ovarian tissue sample of the individual to be diagnosed to the expression level of the corresponding tumor marker in the non-cancerous ovarian tissue sample; and
(4) determining that the individual being diagnosed is affected with ovarian cancer if the tumor markers having the amino acid sequences of SEQ ID NOs: 1-5 are up-regulated and the tumor markers having the amino acid sequences of SEQ ID NOs: 6-13 are down-regulated in the ovarian tissue sample of the individual being diagnosed in comparison with the non-cancerous ovarian tissue sample.

### Claim 2 (depends on 1)

The method of claim 1, wherein the expression levels of the tumor markers are determined by gel electrophoresis.

### Claim 3 (depends on 1)

The method of claim 1, wherein the expression levels of the tumor markers are determined by Western Blot.

### Claim 4 (depends on 1)

The method of claim 1, wherein the expression levels of the tumor markers are determined by Dot blot.

### Claim 5 (depends on 1)

The method of claim 1, wherein the non-cancerous ovarian tissue sample is obtained from the same individual to be diagnosed.

### Claim 6 (depends on 1)

The method of claim 1, wherein the ovarian cancer is stage I, II, III, or IV ovarian cancer.