# US 7,745,123 B2 · Cell cycle reporting cell line

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- Number: US 7,745,123 B2
- Document ID: US7745123B2
- Status: Granted
- Kind: B2
- Application number: 11/572,516
- Filed: 2005-07-22
- Published: 2007-10-11
- Granted: 2010-06-29
- Earliest claimed: 2004-07-23
- Assignees: Ge Healthcare UK Limited; Vanderbilt University
- Inventors: Suzanne Hancock; Simon Stubbs; Nicholas Thomas; Ellen Fanning; Jinming Gu
- Web page: https://patentgrep.com/patent/US7745123B2

## CPC

- G01N 33/5011: {for testing antineoplastic activity}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

The present invention relates to non-destructive and dynamic means for determining the cell cycle position of living cells. The invention provides stable cell lines which can be used to determine the cell cycle position, together with methods for measuring the effect of a test agent on the cell cycle position.

## Claims

### Claim 1

A stable cell line expressing:
i) a first polypeptide construct comprising a first detectable live-cell reporter molecule linked to at least one cell cycle phase-dependent location control element that is a cell cycle phase position motif which is the phosphorylation-dependent subcellular localization domain of the C-terminal special control region of helicase B (PSLD), wherein the location is indicative of the cell cycle position; and
ii) a second polypeptide construct comprising a second detectable live cell reporter molecule such as an enzyme or fluorescent protein linked to a destruction control element that is the cyclin B1 D box, wherein said second reporter is detectable in a mammalian cell at a predetermined position in the cell cycle;
wherein said first and second reporter molecules are chosen to be distinguishable.

### Claim 2 (depends on 1)

The stable cell line of claim 1, wherein the first and second live-cell reporter molecules are selected from the group consisting of fluorescent protein and enzyme reporter.

### Claim 3 (depends on 2)

The stable cell line of claim 2, wherein said fluorescent protein is selected from the group consisting of Green Fluorescent Protein (GFP), Enhanced Green Fluorescent Protein (EGFP), Emerald and J-Red.

### Claim 4 (depends on 2)

The stable cell line of claim 2, wherein the first reporter molecule is EGFP and the second reporter molecule is J-Red, or the first reporter molecule is J-Red and the second reporter molecule is EGFP.

### Claim 5 (depends on 1)

A method for determining the cell cycle position of a mammalian cell, said method comprising:
a) culturing the stable cell line of claim 1; and
b) determining the cell cycle position by monitoring signals emitted by the first and second reporter molecules.

### Claim 6 (depends on 1)

A method for determining the effect of a test agent on the cell cycle position of a mammalian cell, said method comprising:
a) culturing the stable cell line of claim 1; and
b) determining the cell cycle position by monitoring signals emitted by the first and second reporter molecules wherein a difference between the emitted signals measured in the absence and in the presence of said test agent is indicative of the effect of the test agent on the cell cycle position of the cell.

### Claim 7 (depends on 1)

A method for determining the effect of a test agent on the cell cycle position of a mammalian cell, said method comprising:
a) culturing the stable cell line of claim 1;
b) determining the cell cycle position by monitoring signals emitted by the first and second reporter molecules; and
c) comparing the emitted signals in the presence of the test agent with a known value for the emitted signals in the absence of the test agent;
wherein a difference between the emitted signals measured in the presence of the test agent and said known value in the absence of the test agent is indicative of the effect of the test agent on the cell cycle position of the cell.

### Claim 8 (depends on 1)

A method for determining the effect of the mammalian cell cycle on a cellular process monitored by a process reporter which is known to vary in response to a test agent, said method comprising:
a) culturing the stable cell line of claim 1;
b) determining the cell cycle position by monitoring signals emitted by the first and second reporter molecules; and
c) monitoring the signals emitted by the process reporter wherein the process reporter is distinguishable from the first and second reporter molecules;
wherein the relationship between cell cycle position determined by step b) and the signal emitted by the process reporter is indicative of whether or not the cellular process is cell cycle dependent.