# US 7,745,111 B2 · Molecular markers of plant embryogenesis > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 7,745,111 B2 - Document ID: US7745111B2 - Status: Granted - Kind: B2 - Application number: 10/028,346 - Filed: 2001-12-20 - Published: 2010-05-13 - Granted: 2010-06-29 - Earliest claimed: 2000-12-20 - Assignees: Malaysian Palm Oil Board - Inventors: Meilina Ong Abdullah; Harikrishna Kulaveerasingam - Web page: https://patentgrep.com/patent/US7745111B2 ## CPC - C07K 14/415: from plants - A61P 39/06: Free radical scavengers or antioxidants - C12N 9/0065: {acting on hydrogen peroxide as acceptor (1.11)} - C12N 15/8287: {for fertility modification, e.g. apomixis} - C12Q 1/6895: for plants, fungi or algae - G01N 33/53: Immunoassay; Biospecific binding assay; Materials therefor - A01K 2217/05: Animals comprising random inserted nucleic acids (transgenic) - A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00) - C07K 2299/00: Coordinates from 3D structures of peptides, e.g. proteins or enzymes - C12Q 2600/158: Expression markers - Y10T 436/143333: Saccharide [e.g., DNA, etc.] - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - A01K: ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION ## Abstract The present invention relates generally to a molecular marker for a plant physiological process and more particularly for plant embryogenesis. The molecular marker is, in one form, a genetic sequence from a monocot plant such as but not limited to oil-palm plants. In another form, the molecular marker is a polypeptide encoded by said genetic sequence. More particularly, the molecular marker of the present invention enables embryogenic tissue to be detected in vitro. The early detection of embryogenic tissue enables non-embryogenic tissue to be discarded. The ability to detect embryogenesis facilitates maximization of embryogenic potential. The present invention further contemplates a molecular marker comprising in one form a sequence of nucleotides encoding an antioxidant or in another form a sequence of amino acids defining a polypeptide having antioxidant activity. The antioxidant according to this aspect of the present invention is particularly useful in tablet or cream form as an anti-aging agent. The molecular markers of the present invention therefore also have uses in the inhibition or retardation of apoptotic processes. Such an effect has benefits in both plant and animal cells. The present invention further contemplates a promoter sequence encoding the molecular marker and its use in generating male sterile plants. ## Claims ### Claim 1 An isolated nucleic acid molecule encoding a polypeptide comprising the amino acid sequence as set forth in SEQ ID NO: 2. ### Claim 2 (depends on 1) The isolated nucleic acid molecule of claim 1, wherein said nucleic acid molecule comprises the sequence of nucleotides as set forth in SEQ ID NO: 1 or SEQ ID NO: 3 or its complementary form. ### Claim 3 (depends on 1) The isolated nucleic acid molecule of claim 1, wherein the nucleic acid molecule comprises the nucleotide sequence as set forth in SEQ ID NO: 1. ### Claim 4 (depends on 1) The isolated nucleic acid molecule of claim 1, wherein the nucleic acid molecule comprises the nucleotide sequence as set forth in SEQ ID NO: 3. ### Claim 5 A genetic construct comprising a nucleic acid molecule encoding a polypeptide comprising the amino acid sequence as set forth in SEQ ID NO: 2. ### Claim 6 (depends on 5) The genetic construct of claim 5, wherein the nucleic acid molecule comprises the nucleotide sequence as set forth in SEQ ID NO: 1 or SEQ ID NO: 3 or its complementary form. ### Claim 7 (depends on 5) The genetic construct of claim 5, wherein the nucleic acid molecule comprises the sequence of nucleotides as set forth in SEQ D NO: 1. ### Claim 8 (depends on 5) The genetic construct of claim 5, wherein the nucleic acid molecule comprises the sequence of nucleotides as set forth in SEQ ID NO: 3. ### Claim 9 (depends on 5, 6) The genetic construct of claim 5 or 6, wherein said construct further comprises one or more promoter sequences or transcription termination sequences. ### Claim 10 (depends on 9) The genetic construct of claim 9, wherein said construct further comprises one or more origins of replication and/or selectable marker gene sequences. ### Claim 11 (depends on 5, 6, 7, 8) A vector comprising the construct of any one of claims 5-6 or 7-8. ### Claim 12 A host cell comprising an isolated nucleic acid molecule encoding a polypeptide comprising the amino acid sequence as set forth in SEQ ID NO: 2, wherein the isolated nucleic acid molecule is introduced into the host cell. ### Claim 13 (depends on 12) The host cell of claim 12, wherein said nucleic acid molecule comprises the sequence of nucleotides as set forth in SEQ ID NO: 1 or SEQ ID NO: 3 or its complementary form. ### Claim 14 (depends on 12) The host cell of claim 12, wherein the nucleic acid molecule comprises the nucleotide sequence as set forth in SEQ ID NO: 1. ### Claim 15 (depends on 12) The host cell of claim 12, wherein the nucleic acid molecule comprises the nucleotide sequence as set forth in SEQ ID NO: 3. ### Claim 16 (depends on 12) The host cell of claim 12, wherein the cell is a plant cell. ### Claim 17 (depends on 16) The plant cell of claim 16, wherein the cell is from an oil-palm plant. ### Claim 18 (depends on 1, 2) A method for producing a recombinant polypeptide in a host cell or tissue, said method comprising introducing into the said cell or tissue an expression vector comprising the nucleic acid molecule of claim 1 or 2, wherein said nucleic acid molecule is operably linked to one or more regulatory sequences such that the nucleic acid molecule is capable of being expressed in said cell or tissue. ### Claim 19 (depends on 18) The method of claim 18, wherein said nucleic acid molecule comprises the sequence of nucleotides as set forth in SEQ ID NO: 1. ### Claim 20 (depends on 18) The method of claim 18, wherein said nucleic acid molecule comprises the sequence of nucleotides as set forth in SEQ ID NO: 3. ### Claim 21 A method for detecting embryogenic plant material, said method comprising immobilizing a sample containing RNA from the material to be screened on a solid support and contacting said immobilized sample with a labeled nucleic acid molecule which comprises the nucleotide sequence set forth in SEQ ID NO: 1 or SEQ ID NO: 3, and then detecting the presence of said label. ### Claim 22 (depends on 21) A method of claim 21, wherein said nucleotide sequence is as set forth in SEQ ID NO: 1. ### Claim 23 (depends on 21) A method of claim 21, wherein said nucleotide sequence is as set forth in SEQ ID NO: 3.