# US 7,745,107 B2 · Method for assaying flowing media for microbial toxins

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- Number: US 7,745,107 B2
- Document ID: US7745107B2
- Status: Granted
- Kind: B2
- Application number: 10/474,694
- Filed: 2002-03-25
- Published: 2004-08-19
- Granted: 2010-06-29
- Earliest claimed: 2001-04-12
- Inventors: Thomas Hartung
- Web page: https://patentgrep.com/patent/US7745107B2

## CPC

- G01N 33/6869: {Interleukin}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

Testing of flowing media for microbial toxins via whole blood incubation in a flow vessel containing a separation element and assaying for mediators.

## Claims

### Claim 1

A method for testing gas samples or liquid samples for immune-stimulating microbiological substances comprising a) providing a flow through container having a supply opening and an outlet opening, each supply opening and outlet opening having a cover impermeable to microbial substances when closed, and having a separation element or elements inside the flow through container, the separation element or elements comprising filter or filters, b) passing the gas sample or the liquid sample through the supply opening of the flow through container and through the separation element or elements wherein immune-stimulating microbiological substances, if present, in the sample are retained within the filter or filters and wherein the sample exits the container through the outlet opening; c) adding whole blood containing anticoagulants to the surface of separation element or elements thereby contacting the whole blood to the immune-stimulating microbiological substances if present and incubating the filter or filters in the flow through container; d) assaying the whole blood to determine the presence of mediators produced by the whole blood in response to the immune-stimulating microbiological substances if present.

### Claim 2 (depends on 1)

A method in accordance with claim 1, which further comprises a quantitative determination of microbiological substances contaminating the respective test medium.

### Claim 3 (depends on 1)

A method in accordance with claim 1, wherein the separation element contains filter paper or filter fleeces containing organic fibers or porous ion-exchange resins.

### Claim 4 (depends on 1)

A method in accordance with claim 1, wherein the separation element contains cellulose, cellulose ester, polytetrafluoroethylene, polycarbonate and/or glass fibers.

### Claim 5 (depends on 1)

A method in accordance with claim 1, wherein the separation elements have toxin-reactive groups.

### Claim 6 (depends on 1)

A method in accordance with claim 1, wherein the gas is air.

### Claim 7 (depends on 1)

A method in accordance with claim 1, wherein the separation element or elements is arranged in an area between the supply opening and the outlet opening and perpendicularly to the flow of the sample.

### Claim 8 (depends on 1)

A method in accordance with claim 1, wherein the liquid is an aqueous or biological preparation.

### Claim 9 (depends on 8)

A method in accordance with claim 8, wherein the aqueous or biological preparation is a dialysis liquid.