# US 7,744,937 B2 · Chemoprotectants from crucifer seeds and sprouts

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- Number: US 7,744,937 B2
- Document ID: US7744937B2
- Status: Granted
- Kind: B2
- Application number: 11/199,752
- Filed: 2005-08-09
- Published: 2007-02-08
- Granted: 2010-06-29
- Earliest claimed: 2005-08-09
- Assignees: Kraft Foods Global Brands LLC
- Inventors: Leslie G. West; George W. Haas; Nathan Matusheski
- Web page: https://patentgrep.com/patent/US7744937B2

## CPC

- A23L 33/105: Plant extracts, their artificial duplicates or their derivatives
- A61P 3/02: Nutrients, e.g. vitamins, minerals
- A61P 35/00: Antineoplastic agents
- A61P 39/06: Free radical scavengers or antioxidants
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

The present invention is directed to chemoprotectant precursor compositions provided from crucifer seeds and sprouts and methods for their preparation. Treatment of aqueous extracts from crucifer seeds or sprouts with adsorbents substantially increases the ratio of certain highly chemoprotectant precursor compounds (alkyl glucosinolates such as glucoraphanin, a.k.a. sulforaphane glucosinolate) to undesirable compounds such as indole glucosinolates (for example 4-hydroxyglucobrassicin). The method provides an extract which has a ratio of glucoraphanin to 4-hydroxyglucobrassicin of about 70 or greater. The resulting extract has improved color and odor and may be dried or used directly as an additive in a variety of foodstuffs.

## Claims

### Claim 1

A method for extracting chemoprotectant precursors comprising:
forming an aqueous extract of broccoli seeds or sprouts;
contacting the aqueous extract with about 8 to about 12 weight percent of activated carbon; and
separating the activated carbon from the aqueous extract to provide a chemoprotectant precursor enhanced extract,
the method effective for providing a chemoprotectant precursor enhanced extract having a ratio of a number of alkyl glucosinolates to indole glucosinolates of at least about 30.

### Claim 2 (depends on 1)

The method of claim 1, wherein the alkyl glucosinolates are glucoraphanin.

### Claim 3 (depends on 1)

The method of claim 1, wherein the indole glucosinolates are 4-hydroxyglucobrassicin.

### Claim 4 (depends on 1)

The method of claim 1, wherein the ratio of number of alkyl glucosinolates to indole glucosinolates is about 30 to about 1000.

### Claim 5 (depends on 1)

The method of claim 1, wherein the ratio of alkyl glucosinolates to indole glucosinolates is about 1000 or greater.

### Claim 6 (depends on 1)

The method of claim 1, wherein the broccoli seeds are defatted prior to forming an aqueous extract.

### Claim 7 (depends on 1)

The method of claim 1, wherein the aqueous extract of broccoli seeds or sprouts is formed by contacting the broccoli seeds or sprouts with water having a temperature of about 60° to 110° C. for at least about 5 minutes.