Methods, apparatus and computer program products for formulating culture media

A method of identifying a peptide with a desired activity having an indicia that satisfies a test requirement, the method comprising the steps of:

• identifying a predetermined set of peptides;
• parameterizing the predetermined set of peptides by:
• determining a first parameter for each predetermined peptide, wherein the first parameter is a whole molecule parameter, and
• determining a second parameter for each predetermined peptide, wherein the second parameter is dependent on the specific order of constitutive subunits within each predetermined peptide;
• performing a space-filling design of the parameterized peptides to identify first test peptides;
• constructing a first test peptide library comprising a plurality of first test peptides identified using the space-filling design, wherein the length of said first test peptides comprises about four amino acids to about twenty amino acids, and wherein said first test peptides are a subset of said predetermined set of peptides;
• determining an activity, having an indicia, of said plurality of first test peptides;
• measuring the indicia of said activity for said plurality of first test peptides;
• deriving a quantitative relationship between said indicia of said activity, said first parameter, and said second parameter;
• setting a test requirement, based on a desired activity, having a test indicia range;
• selecting a subgroup of first test peptides having an indicia that satisfies said test requirement;
• and
• expanding first test peptides from said subgroup into their constituent compound isomers;
• performing a space-filling design on said constituent compound isomers to identify candidate peptides;
• calculating an estimated indicia for each candidate peptide using said quantitative relationship;
• selecting a second test peptide library comprising at least one second test peptide, wherein each second test peptide is a candidate peptide having an estimated indicia that satisfies said test requirement, and wherein said second test peptides are not in said first test peptide library;
• measuring the indicia of each second test peptide;
• and
• identifying at least one second test peptide having a measured indicia that satisfies said test requirement.

A method of identifying a peptide with a desired activity having an indicia that satisfies a test requirement, the method comprising the steps of:

• identifying a plurality of initial peptides having a length of about four amino acids to about twenty amino acids;
• parameterizing the initial peptides by:
• determining a first parameter for each initial peptide, wherein the first parameter is a whole molecule parameter, and
• determining a second parameter for each initial peptide, wherein the second parameter is dependent on the specific order of constitutive subunits within each initial peptide;
• performing a space-filling design of the parameterized peptides to identify first test peptides;
• constructing a first test peptide library comprising a plurality of test peptides identified using the space-filling design, wherein said first test peptides are a subset of said initial peptides;
• measuring the indicia of an activity of said plurality of test peptides;
• deriving a quantitative relationship between said indicia of said activity, said first parameter, and said second parameter of said plurality of test peptides;
• calculating an estimated indicia for each initial peptide using said quantitative relationship;
• setting a test requirement, based on a desired activity, said test requirement having a test indicia range;
• selecting a second test peptide library comprising at least one second test peptide, wherein each second test peptide has an estimated indicia that satisfies said test requirement, and wherein said second test peptides are not in said first test peptide library;
• measuring the indicia of each second test peptide;
• and
• identifying at least one second test peptide having a measured indicia that satisfies said test requirement.

A method of identifying a peptide with a desired activity having an indicia that satisfies a test requirement, the method comprising the steps of:

• identifying a predetermined set of peptides;
• parameterizing the predetermined set of peptides by:
• determining a first parameter for each predetermined peptide, wherein the first parameter is a whole molecule parameter, and
• determining a second parameter for each predetermined peptide, wherein the second parameter is dependent on the specific order of constitutive subunits within each predetermined peptide;
• performing a space-filling design of the parameterized peptides to identify first test peptides;
• constructing a first test peptide library comprising a plurality of first test peptides identified using the space-filling design, wherein said first test peptides are a subset of said predetermined set of peptides;
• determining an activity, having an indicia, of said plurality of first test peptides;
• measuring the indicia of said activity for said plurality of first test peptides;
• deriving a quantitative relationship between said indicia of said activity, said first parameter, and said second parameter;
• calculating an estimated indicia for each remaining peptide from said predetermined set of peptides using said quantitative relationship;
• setting a test requirement, based on a desired activity, having a test indicia range;
• selecting a second test peptide library comprising at least one second test peptide, wherein each second test peptide has an estimated indicia that satisfies said test requirement, and wherein said second test peptides are not in said first test peptide library;
• measuring the indicia of each second test peptide;
• and
• identifying at least one second test peptide having a measured indicia that satisfies said test requirement.