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US 7,741,859 B2Granted
Detection of sealing by means of noise analysis
AssigneeMax-Planck-Gesellschaft zur Foerderung der Wissenschaften e.V.
InventorMoritz Völker
Life of this patent
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Abstract
The invention relates to a method for determining the sealing of a substance on a support by determining the electrical noise.
Claims38
A method for determining the sealing of a substance on a support, comprising
- (a) providing a substance on a support comprising at least one first electrode in contact with an electrically conductive liquid, wherein the substance entirely or partially covers the at least one first electrode,
- (b) determining the electrical noise of the sealing impedance between the at least one first electrode and a second electrode independently from external voltage, wherein essentially no current flows via the boundary surface of the at least one first electrode and the second electrode to the electrically conductive liquid, and
- (c) determining the sealing of the substance on the support from the electrical noise.
A method for identifying a compound which modulates the sealing of a substance on a support, comprising:
- (A)
- (I)
- determining the sealing of the substance on a support by a method (X) which comprises
- (a) providing a substance on a support comprising at least one first electrode in contact with an electrically conductive liquid, wherein the substance entirely or partially covers the at least one first electrode,
- (b) determining the electrical noise of the impedance between the at least one first electrode and a second electrode, wherein essentially no current flows via the boundary surface of the at least one first electrode and the second electrode to the electrically conductive liquid, and
- (c) determining the sealing of the substance on the support from the electrical noise,
- and/or
- using an apparatus (XX) comprising
- (i) a support for holding a substance, comprising at least one first electrode in contact with an electrically conductive liquid,
- (ii) means for measuring the electrical noise of the impedance between the at least one first electrode and a second electrode independently from external voltage and wherein essentially no current flows via the boundary surface of the at least one first electrode and the second electrode to the electrically conductive liquid, and
- (iii) means for determining the sealing of the substance on the support from the electrical noise, in the absence of the compound to be investigated,
- (II)
- determining the sealing of the substance on a support using the method (X) and/or using said apparatus (XX) in the presence of the compound to be investigated, and
- (III)
- selecting a compound which modulates the sealing of the substance on the support in a predetermined manner;
- or
- (B)
- (1)
- determining the adhesion of the substance on a support by a method (Y) which comprises
- (a) providing a substance on a support comprising at least one first electrode in contact with an electrically conductive liquid, wherein the substance entirely or partially covers the at least one first electrode,
- (b) determining the electrical noise of the impedance between the at least one first electrode and a second electrode, wherein essentially no current flows via the boundary surface of the at least one first electrode and the second electrode to the electrically conductive liquid,
- (c) determining the sealing of the substance on the support from the electrical noise, and
- (d) determining the adhesion of the substance on the support from the sealing of the substance on the support,
- and/or
- using an apparatus (YY) comprising
- (i) a support for holding a substance, comprising at least one first electrode in contact with an electrically conductive liquid,
- (ii) means for measuring the electrical noise of the impedance between the at least one first electrode and a second electrode independently from external voltage and wherein essentially no current flows via the boundary surface of the at least one first electrode and the second electrode to the electrically conductive liquid, and
- (iii) means for determining the sealing of the substance on the support from the electrical noise, and
- (iv) means for determining the adhesion of the substance on the support from the sealing of the substance on the support,
- in the absence of the compound to be investigated,
- (2)
- determining the adhesion of the substance on a support using the method (Y) and/or using said apparatus (YY) in the presence of the compound to be investigated, and
- (3)
- selecting a compound which modulates the adhesion of the substance on the support in a predetermined manner.
31 more elements
In a method for diagnosis of a malignant tumor, wherein the improvement comprises determining the sealing of a malignant tumor cell on a support, which determination comprises
- (a) providing a malignant tumor cell on a support comprising at least one first electrode in contact with an electrically conductive liquid, wherein the malignant tumor cell entirely or partially covers the at least one first electrode,
- (b) determining the electrical noise of the sealing impedance between the at least one first electrode and a second electrode independently from external voltage, wherein essentially no current flows via the boundary surface of the at least one first electrode and the second electrode to the electrically conductive liquid, and
- (c) determining the sealing of the malignant tumor cell on the support from the electrical noise.
A method for determining the sealing of a substance on a support, comprising
- (a)providing a substance on a support comprising at least one first electrode in contact with an electrically conductive liquid, wherein the substance entirely or partially covers the at least one first electrode,
- (b) determining the electrical noise of the impedance as a noise voltage between the at least one first electrode and a second electrode, and
- (c) determining the sealing of the substance on the support from the electrical noise (a) from the amplitude of the noise voltage in a predetermined frequency range f1 to f2, and wherein the amplitude is characterized by the voltage range in which the electrical noise voltage moves for up to 95% of a predetermined time period, or (b) from the amplitude of the noise voltage in a predetermined frequency range f1 to f2, and wherein the amplitude of the noise voltage is characterized by ±3.2 σ.
Dependencies are inferred from the claim text.