Diacylglycerol acyl transferase proteins
AssigneeMonsanto Technology LLC
InventorsKathryn Dennis Lardizabal, Gregory A. Thompson, Deborah Hawkins
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Abstract
The invention provides diacylglycerol acyltransferase (DAGAT) proteins, wherein said proteins are active in the formation of triacylglycerol from fatty acyl and diacylglycerol substrates. In one aspect, Mortierella ramanniana DAGAT proteins have been isolated and have molecular weights of between approximately 36 and 37 kDa as measured by SDS-PAGE. The invention also provides novel DAGAT polynucleotide and polypeptide sequences and to methods of producing such polypeptides using recombinant techniques. In addition, methods are provided for using such sequences to alter triacylglycerol levels in plants and to treat diseases associated with altered DAGAT activity or expression.
Claims21
An isolated polynucleotide that encodes a polypeptide with diacylglycerol acyltransferase activity and is selected from the group consisting of:
- a) an isolated polynucleotide comprising SEQ ID NO:
- 37;
- b) an isolated polynucleotide comprising a nucleotide sequence which has at least 70% identity to that of SEQ ID NO:
- 37 over the entire length of SEQ ID NO:
- 37;
- c) an isolated polynucleotide that hybridizes, under stringent conditions, to SEQ ID NO:
- 37 or a fragment thereof;
- d) an isolated polynucleotide comprising a nucleotide sequence which has at least 70% identity to that of SEQ ID NO:
- 84 over the entire length of SEQ ID NO:
- 84;
- e) an isolated polynucleotide that hybridizes, under stringent conditions, to SEQ ID NO:
- 84 or a fragment thereof;
- and
- f) an isolated polynucleotide complementary to the polynucleotide sequence of (a), (b), (c), (d), or (e).
Dependencies are inferred from the claim text.