Non-fluorescent energy transfer
AssigneeRoche Molecular Systems, Inc.
InventorStephen G. Will
Life of this patent
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Abstract
The present invention relates generally to the transfer of non-fluorescent energy between donor and acceptor moieties. In certain embodiments, the invention provides biomolecules that include substantially non-fluorescent donor moieties. Processes involving these donor moieties typically entail reduced background fluorescence relative to applications that involve conventional fluorescent donor moieties. In addition to reaction mixtures and methods that include the use of these biomolecules, the invention also provides related kits and systems.
Claims23
A biomolecule comprising a single substantially non-fluorescent donor moiety that is capable of transferring non-fluorescent energy to at least one acceptor moiety when the acceptor moiety is sufficiently proximal to the substantially non-fluorescent donor moiety such that the acceptor moiety emits light in response to the accepted non-fluorescent energy;
- wherein the substantially non-fluorescent donor moiety is selected from the group consisting of:
- 6-carboxy-aminopentachiorofluorescein, or 5-carboxy-aminopentachiorofluorescein.
A reaction mixture, comprising at least one nucleotide, at least one primer nucleic acid, and/or at least a first probe nucleic acid, wherein one or more of the nucleotide, the primer nucleic acid, or the first probe nucleic acid comprises a single substantially non-fluorescent donor moiety that is capable of transferring non-fluorescent energy to at least one acceptor moiety when the acceptor moiety is sufficiently proximal to the substantially non-fluorescent donor moiety such that the acceptor moiety emits light in response to the accepted non-fluorescent energy;
- wherein the substantially non-fluorescent donor moiety is selected from the group consisting of:
- 6-carboxy-aminopentachlorofluorescein, or 5-carboxy-aminopentachlorofluorescein.
A reaction mixture comprising at least a first biopolymer synthesis reagent that comprises a single substantially non-fluorescent donor moiety that is capable of transferring non-fluorescent energy to at least one acceptor moiety when the acceptor moiety is sufficiently proximal to the substantially non-fluorescent donor moiety such that the acceptor moiety emits light in response to the accepted non-fluorescent energy and the substantially non-fluorescent donor moiety is selected from the group consisting of:
- 6-carboxy-aminopentachlorofluorescein, or 5-carboxy-aminopentachiorofluorescein.
A kit, comprising at least a first biomolecule comprising a single substantially non-fluorescent donor moiety that is capable of transferring non-fluorescent energy to at least one acceptor moiety when the acceptor moiety is sufficiently proximal to the substantially non-fluorescent donor moiety such that the acceptor moiety emits light in response to the accepted non-fluorescent energy and the substantially non-fluorescent donor moiety is selected from the group consisting of:
- 6-carboxy-aminopentachlorofluorescein, or 5-carboxy-aminopentachlorofluorescein.
Dependencies are inferred from the claim text.