Polypeptides having cellulolytic enhancing activity and nucleic acids encoding same
AssigneeNovozymes, Inc.
InventorsKimberly Brown, Paul Harris, Elizabeth Zaretsky, Edward Re, Elena Vlasenko, Keith McFarland, Alfredo Lopez de Leon
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Abstract
The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
Claims23
An isolated polynucleotide comprising a nucleotide sequence which encodes a polypeptide having cellulolytic enhancing activity, wherein the isolated polynucleotide is selected from the group consisting of:
- (a) a polynucleotide encoding a polypeptide having cellulolytic enhancing activity comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
- 2;
- (b) a polynucleotide encoding a polypeptide having cellulolytic enhancing activity comprising a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 1;
- and
- (c) a polynucleotide encoding a polypeptide having cellulolytic enhancing activity comprising a nucleotide sequence that hybridizes under at least high stringency conditions with a full-length complementary strand of SEQ ID NO:1 or the mature polypeptide coding sequence of SEQ ID NO:1, wherein the high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA and 50% formamide, and washing three times each for 15 minutes using 2×SSC and 0.2% SDS at 65° C.
An isolated polynucleotide that comprises the nucleotide sequence of SEQ ID NO:
- 1, or a subsequence thereof encoding a polypeptide fragment having cellulolytic enhancing activity.
An isolated polynucleotide that comprises a nucleotide sequence the full length of which hybridizes under at least high stringency conditions with (i) the full-length mature polypeptide coding sequence of SEQ ID NO:
- 1, (ii) the full-length cDNA sequence contained in the mature polypeptide coding sequence of SEQ ID NO:
- 1, or (iii) a full-length complementary strand of (i) or (ii), wherein the high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA and 50% formamide, and washing three times each for 15 minutes using 2×SSC and 0.2% SDS at 65° C.
An isolated polynucleotide that comprises a nucleotide sequence the full length of which hybridizes under at least very high stringency conditions with (i) the full-length mature polypeptide coding sequence of SEQ ID NO:
- 1, (ii) the full-length cDNA sequence contained in the mature polypeptide coding sequence of SEQ ID NO:
- 1, or (iii) a full-length complementary strand of (i) or (ii), wherein the very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA and 50% formamide, and washing three times each for 15 minutes using 2×SSC and 0.2% SDS at 70° C.
An isolated polynucleotide that consists of the nucleotide sequence of SEQ ID NO:1 or that consists of a fragment of the nucleotide sequence of SEQ ID NO:
- 1, the fragment encoding a polypeptide having cellulolytic enhancing activity.
Dependencies are inferred from the claim text.