Feline hemoplasma isolate
AssigneeThe University of Zurich Veterinary Clinical Laboratory
InventorsBarbara Willi, Regina Hofmann-Lehmann, Hans Lutz, Felicitas S. Boretti
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Abstract
A newly identified hemoplasma agent, Candidatus Mycoplasma turicensis, is disclosed. Also disclosed are detection methods, screening methods and methods of diagnosis for the hemoplasma agent.
Claims18
A purified nucleic acid molecule consisting of SEQ ID NO:
- 3 or 4 and optionally an isotopic label, non-isotopic label, a catalyst label, an enzyme label, a promoter label, a dye label, a fluorescent molecule, a chemiluminescent label, a coenzyme, an enzyme substrate, a radioactive group, a small organic molecule, an amplifiable polynucleotide sequence label, a particle label, a metal sol label, a crystallite label, a liposome label, a cell label, or a colorimetric label.
A method of detecting 16 S rRNA nucleic acid molecules of a hemoplasma agent in a sample comprising:
- amplifying the 16S rRNA nucleic acid molecules of the hemoplasma agent using a first amplification primer consisting of SEQ ID NO:3 and a second amplification primer consisting of SEQ ID NO:4 ;
- and detecting an amplification product, wherein if an amplification product is detected, the 16S rRNA nucleic acid molecule is present.
A method for detecting and quantifying nucleic acid molecules of a hemoplasma agent comprising:
- amplifying a 16S rRNA sequence of the hemoplasma agent using a first primer consisting of SEQ ID NO:3 ;
- a second primer consisting of SEQ ID NO:4 ;
- a DNA polymerase comprising 5′ nuclease activity;
- a nucleic acid probe comprising nucleic acids complementary to the 16S rRNA sequence and comprising a reporter fluorescent dye and a quencher dye;
- wherein the nucleic acid from the hemoplasma agent is detected and quantified.
A kit for detecting a nucleic acid molecule, comprising purified nucleic acid molecules consisting of SEQ ID NO:3 and consisting of SEQ ID NO:4.
An isolated hemoplasma agent deposited as ATCC PTA-6782.
An isolated hemoplasma agent, wherein a polymerase chain reaction (PCR) performed using nucleic acids of the hemoplasma agent with PCR primers consisting of SEQ ID NO:3 and SEQ ID NO:4 results in an amplification product, and wherein the hemoplasma agent comprises a 16S rRNA sequence of SEQ ID NO:12.
Dependencies are inferred from the claim text.