Polynucleotides encoding a truncated ORF2 from Corynebacterium
AssigneeArcher Daniels Midland Company
InventorsPaul D. Hanke, Lhing-Yew Li-D'Elia, Holly J. Walsh
Life of this patent
Estimating the term…
Abstract
The invention provides methods to increase the production of an amino acid from Corynebacterium species by way of the amplification of amino acid biosynthetic pathway genes in a host cell chromosome. Amplification may be by integration of one or more copies of a gene or genes into a host cell chromosome. One gene that may be incorporated is the gene ORF2, which encodes an unnamed hypothetical protein and which may be obtained from Corynebacterium glutamicum. The invention also provides novel isolated nucleic acid molecules for L-lysine biosynthetic pathway genes of Corynebacterium glutamicum.
Claims15
An isolated polynucleotide molecule comprising:
- (i) a nucleotide sequence encoding the polypeptide sequence of SEQ ID NO:
- 19;
- and
- (ii) a nucleotide sequence encoding the polypeptide sequence of SEQ ID NO:
- 2.
A method for selecting a transformed host cell comprising:
- (a) transforming a Corynebacterium species host cell with a vector comprising a polynucleotide molecule comprising a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:
- 19 and the amino acid sequence of SEQ ID NO:
- 2, wherein following transformation said polynucleotide molecule is integrated into the chromosome of said host cell, and
- (b) selecting a transformed host cell.
Dependencies are inferred from the claim text.