Binding molecules for the treatment of myeloid cell malignancies
AssigneeCrucell Holland B.V.
InventorsSonja van den Oudenrijn, Marja van Meijer, Adrianus Q. Bakker, Alexander Berthold Hendrik Bakker
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Abstract
The present invention provides a human C-type lectin, binding molecules that specifically bind to the human C-type lectin, nucleic acid molecules encoding the binding molecules or the human C-type lectin, compositions comprising the binding molecules or the human C-type lectin and methods of identifying or producing the binding molecules. The human C-type lectin is specifically expressed on myeloid cells and binding molecules capable of specifically binding to the human C-type lectin can be used in the diagnosis, prevention and/or treatment of neoplastic disorders and diseases.
Claims16
An isolated antibody capable of specifically binding to a human myeloid C-type lectin, wherein the antibody comprises a heavy chain variable region comprising the variable region of the amino acid sequence selected from the group consisting of SEQ ID NO:7, SEQ ID NO:9, and SEQ ID NO:11, and a light chain variable region comprising the variable region of the amino acid sequence of SEQ ID NO:13.
An isolated antibody that specifically binds a human C-type lectin, wherein the antibody comprises a heavy chain comprising the amino acid sequence selected from the group consisting of SEQ ID NO:7, SEQ ID NO:9, and SEQ ID NO:11.
An immunoconjugate comprising:
- a antibody comprising:
- a heavy chain variable region comprising a variable region selected from the group of variable regions consisting of SEQ ID NO:7, SEQ ID NO:9, and SEQ ID NO:11, and
- a light chain variable region comprising the amino acid sequence of SEQ ID NO:13, and at least one tag.
A method of detecting a human C-type lectin comprising the amino acid sequence YXXM, wherein X may be any amino acid residue, wherein the method comprises the steps of:
- (a) contacting a sample with a diagnostically effective amount of a antibody comprising:
- a heavy chain variable region comprising a variable region selected from the group of variable regions consisting of SEQ ID NO:7, SEQ ID NO:9, and SEQ ID NO:
- 11, and
- a light chain variable region comprising the amino acid sequence of SEQ ID NO:13, and
- (b) determining whether the antibody specifically binds to a compound of the sample.
A method for selecting myeloid cells comprising the steps of:
- (a) contacting a sample comprising myeloid cells with a antibody comprising:
- a heavy chain variable region comprising a variable region selected from the group of variable regions consisting of SEQ ID NO:7, SEQ ID NO:9, and SEQ ID NO:11, and
- a light chain variable region comprising the amino acid sequence of SEQ ID NO:
- 13,
- (b) separating myeloid cells that bind to the antibody from myeloid cells that do not bind to the antibody, and
- (c) recovering the myeloid cells that are bound by the antibody.
Dependencies are inferred from the claim text.