Method for producing type IV collagen
AssigneeFUJIFILM Corporation
InventorsTakayuki Kobayashi, Tasuku Sasaki, Hayato Miyoshi
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Abstract
An object of the invention is to provide type IV collagen without contamination by other proteins and without degradation or denaturation. The present invention provides a type IV collagen which is derived and extracted from lens capsules without the use of an enzyme and has a minimum molecular weight of 160 to 180 kDa measured by SDS-PAGE under reduced conditions.
Claims6
A method for producing a type IV collagen which is derived and extracted from lens capsule without the use of an enzyme and has a minimum molecular weight of 160 to 180 kDa measured by SDS-PAGE under reduced conditions, wherein collagen is extracted from lens capsules of an animal without the use of an enzyme, which comprises at least steps of:
- (1) agitating lens capsules in a phosphate buffered saline to recover a precipitate;
- (2) agitating the precipitate obtained in step (1) in an aqueous acidic solution to recover a supernatant containing type IV collagen;
- and (3) adding salt to the supernatant obtained in step (2) to allow type IV collagen to precipitate and recovering the resulting precipitate.
Dependencies are inferred from the claim text.