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US 7,741,301 B2Granted
Transposase and method of gene modification
AssigneeJapan Science and Technology Agency
InventorKoichi Kawakami
Life of this patent
Estimating the term…
Abstract
A transposase encoded by the Tol2 element; a polynucleotide encoding the same; a method of modifying the gene structure of a cell (preferably a vertebrate cell) by using the above protein; a method of modifying the function of a cell by modifying the gene structure thereof; and a cell having been modified in function by these methods. Also the structure of a cis element necessary in gene transfer is clarified and presented.
Claims6
A method for modifying a genome structure of a cell, the method comprising a) introducing a nucleic acid encoding a protein having the amino acid sequence of SEQ ID NO:
- 2, and a polynucleotide sequence comprising an exogenous nucleic acid and a cis-element of Oryzias Latipes Tol2 element into the cell;
- b) producing the protein in the cell;
- and c) modifying the genome structure by the insertion of the exogenous nucleic acid into the genome.
Dependencies are inferred from the claim text.