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US 7,741,285 B2Granted
APO-2 ligand/trail formulations
AssigneeGenentech, Inc.
InventorsHeather Flores, Tanya P. Lin, Timothy C. Matthews, Roger Pai, Zahra Shahrokh
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Abstract
The present invention relates generally to Apo2L/TRAIL purification involving crystallization.
Claims30
A method of recovering Apo2L/TRAIL from a mixture comprising
- (a) loading the mixture on a cation exchange column;
- (b) washing the cation exchange column with an equilibration buffer whereby non-binding components present in the mixture are removed;
- (c) eluting Apo2L/TRAIL bound to the cation exchange column with an elution buffer;
- (d) crystallizing Apo2L/TRAIL from the eluate obtained in step (c), wherein said crystallization consists of gradually cooling the eluate to a temperature of about 2 to 8° C., in the presence or absence of an anti-solvent, whereby Apo2L/TRAIL is spontaneously precipitated in a crystalline form to yield a mixture of mother liquor and Apo2L/TRAIL crystals, and
- (e) recovering Apo2L/TRAIL from the mixture obtained in step (d) in a purity of at least about 99%.
2 more elements
Dependencies are inferred from the claim text.