Modified proteins, designer toxins, and methods of making thereof
AssigneeResearch Development Foundation
InventorsMichael G. Rosenblum, Lawrence Cheung
Life of this patent
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Abstract
The present invention concerns methods of reducing the antigenicity of a proteinaceous compound while maintaining the compounds biological activity, as well as proteinaceous compositions with biological activity but reduced antigenicity. These methods and compositions have significant benefits to a subject in need of such compounds and compositions. Also included are modified toxin compounds that are truncated and/or possess reduce antigenicity. Such designer toxins have therapeutic, diagnostic, and preventative benefits, particularly as immunotoxins. Methods of treating cancer using these immunotoxins are provided.
Claims44
A method of killing a cancer cell comprising providing to the cell an effective amount of an immunotoxin comprising a recombinant getonin toxin and single chain antibody that specifically targets the cancer cell, wherein the recombinant gelonin toxin is modified relative to full-length, wild-type gelonin through the removal of one or more sequence regions that are antigenic in humans.
A method of treating cancer in a patient comprising administering to the patient an effective amount of a composition comprising an immunotoxin comprising a recombinant gelonin toxin and single chain antibody that specifically targets a cancer cell, wherein the recombinant gelonin toxin is modified relative to full-length, wild-type gelonin through the removal of one or more sequence regions that are antigenic in humans.
A method for treating cancer in a patient comprising administering to the patient an effective amount of a composition comprising a ligand that selectively targets cells of the patient's cancer, wherein the ligand is conjugated or fused to a recombinant gelonin toxin, wherein the recombinant gelonin toxin is modified relative to full-length, wild-type gelonin through the removal of one or more sequence regions that are antigenic in humans.
A method of treating cancer in a patient comprising administering to the patient an effective amount of a composition comprising an immunotoxin comprising a recombinant gelonin toxin and single chain antibody that specifically targets a cancer cell, wherein the immunotoxin is scfvMEL-2018 having the sequence of SEQ ID NO:
- 11.
A method of killing a cancer cell comprising providing to the cell an effective amount of an immunotoxin comprising a recombinant getonin toxin and single chain antibody that specifically targets the cancer cell, wherein the recombinant gelonin toxin is modified relative to full-length, wild-type gelonin through the replacement of one or more sequence regions that are antigenic in humans with an amino acid sequence that is less antigenic in humans.
A method of treating cancer in a patient comprising administering to the patient an effective amount of a composition comprising an immunotoxin comprising a recombinant gelonin toxin and single chain antibody that specifically targets a cancer cell, wherein the recombinant gelonin toxin is modified relative to full-length, wild-type gelonin through the replacement of one or more sequence regions that are antigenic in humans with an amino acid sequence that is less antigenic in humans.
A method for treating cancer in a patient comprising administering to the patient an effective amount of a composition comprising a ligand that selectively targets cells of the patient's cancer, wherein the ligand is conjugated or fused to a recombinant gelonin toxin, wherein the recombinant gelonin toxin is modified relative to full-length, wild-type gelonin through the replacement of one or more sequence regions that are antigenic in humans with an amino acid sequence that is less antigenic in humans.
Dependencies are inferred from the claim text.