Luminescent markers
AssigneesChristopher Hogue, Susanna Sroka
InventorsChristopher Hogue, Susanna Sroka
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Abstract
The invention relates to luminescent proteins, nucleic acids encoding same, compositions and combinations comprising the proteins, and methods using the proteins, nucleic acids, compositions and combinations. In particular, a luminescent protein is provided comprising oncomodulin in which a salt bridge has been introduced to provide greater stability. The protein may be used as a luminescent marker in, for example, luminescent items, immunoassays, and fluorescent energy transfer assays.
Claims29
A luminescent protein comprising oncomodulin in which:
- (a) Gln27 of oncomodulin is replaced with Lys27;
- (b) Ser36 of oncomodulin is replaced with Lys36;
- (c) C-terminus has been extended;
- (d) one or more salt bridges and/or hydrogen bonding networks have been introduced to provide greater stability when compared to a native oncomodulin or CDOM33;
- and
- (e) Tb3+ luminescence is enhanced relative to CDOM33.
A luminescent protein comprising oncomodulin comprising the amino acid sequence of SEQ ID NO. 5 in which one or more salt bridges and/or hydrogen bonding networks have been introduced to provide greater stability when compared to a native oncomodulin or CDOM33.
A luminescent protein comprising oncomodulin in which Gln27 is replaced with Lys27, Ser36 is replaced with Lys36;
- and a C-terminus is extended.
Dependencies are inferred from the claim text.