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US 7,741,128 B2Granted
Cooperative reporter systems, components, and methods for analyte detection
AssigneeUniversity of Hawaii
InventorWei Wen Su
Life of this patent
Estimating the term…
Abstract
The present invention relates to methods and compositions to detect analytes in a sample using antibody molecules that are transformed into nanoscale “self-signaling” biosensors. It relates in particular to those methods and compositions that provide for single-step detection of target analytes without the need for labor-intensive steps necessary in conventional assays.
Claims19
A method of sensing an analyte, comprising the steps of
- providing a biological sample;
- contacting the sample with at least one reporter reagent for detecting an analyte, wherein the reporter reagent comprises an affinity component, a linking component, and a reporter component;
- determining presence or absence of a signal generated from the contacting, wherein the signal is indicative of presence of the analyte in the sample;
- and
- sensing the analyte based upon presence or absence of the generated signal wherein the signal is not detected above background in the absence of the analyte,
- wherein the linking component comprises a Protein L linker that comprises Protein L or a fragment or derivative thereof, and
- wherein the Protein L linker comprises a modification permitting or enhancing chemical attachment of the linker to the affinity component.
4 more elements
A method of sensing an analyte, comprising the steps of
- providing a biological sample;
- contacting the sample with at least one reporter reagent for detecting an analyte, wherein the reporter reagent comprises an affinity component, a linking component, and a reporter component;
- determining presence or absence of a signal generated from the contacting, wherein the signal is indicative of presence of the analyte in the sample;
- and
- sensing the analyte based upon presence or absence of the generated signal wherein the signal is not detected above background in the absence of the analyte,
- wherein the linking component comprises a Protein L linker that comprises Protein L or a fragment or derivative thereof, and
- wherein the Protein L linker comprises a polypeptide encoded by a polynucleotide having the sequence of SEQ ID NO:
- 2.
5 more elements
A method of sensing an analyte, comprising the steps of
- providing a biological sample;
- contacting the sample with at least one reporter reagent for detecting an analyte, wherein the reporter reagent comprises an affinity component, a linking component, and a reporter component;
- determining presence or absence of a signal generated from the contacting, wherein the signal is indicative of presence of the analyte in the sample;
- and
- sensing the analyte based upon presence or absence of the generated signal wherein the signal is not detected above background in the absence of the analyte,
- wherein the linking component comprises a Protein L linker that comprises Protein L or a fragment or derivative thereof, and
- wherein linkage of the Protein L linker to the affinity component comprises photocrosslinking.
4 more elements
Dependencies are inferred from the claim text.