Detection procedures for fibrinogen and/or fibrinogen derivatives
OwnerThomas W. Stiefinventor
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Abstract
Described are determination procedures for fibrinogen and/or fibrinogen-derivatives by matrix-independent turbidimetry. In the FIFTA called procedure the fibrinogen activity is preferably determined in an undiluted sample by addition of thrombin and/or albumin, as well as polybrene if appropriate, in PBS and determination of the increase in absorbance at 405 nm. In the FIATA called procedure the fibrinogen-concentration and/or the concentration of fibrinogen-derivatives is preferably determined by addition of vancomycin and determination of the increase in turbidity at 405 nm. It is standardized by usage of plasma standards of known fibrinogen-activity and/or fibrinogen-concentration.
Claims9
A method to determine the concentration of fibrinogen and/or derivatives of fibrinogen in a solution, the method comprising:
- (a) measuring the absorbance of the solution;
- (b) adding vancomycin to the solution;
- (c) allowing the vancomycin and solution to react for between two seconds and ten minutes to form a mixture;
- (d) measuring the absorbance of the mixture;
- (e) determining the difference in absorbance between the solution and the mixture;
- (f) comparing the difference in absorbance against a calibration of absorbance for known concentrations of fibrinogen or fibrinogen derivatives to determine the concentration of fibrinogen and/or derivatives of fibrinogen in the solution, wherein the method is independent of matrix and/or independent of time of coagulation.
Dependencies are inferred from the claim text.