Microfluidic device with thin-film electronic devices
AssigneeHewlett-Packard Development Company, L.P.
InventorsGrant Pease, Adam L Ghozeil, John Stephen Dunfield, Winthrop D. Childers, David Tyvoll, Douglas A. Sexton, Paul Crivelli
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Abstract
A microfluidic device for analysis of a sample. The microfluidic device includes a substrate portion that at least partially defines a chamber for receiving the sample. The substrate portion includes a substrate having a surface. The substrate portion also includes a plurality of thin-film layers formed on the substrate adjacent the surface. The thin-film layers form a plurality of electronic devices. Each of at least two of the electronic devices is formed by a different set of the thin-film layers.
Claims18
A method of using a microfluidic device to analyze a biological sample, comprising:
- introducing a biological sample into a chamber;
- and
- operating at least two thin-film electronic devices such that each of the electronic devices senses or modifies a property of the biological sample selectively in a region of the chamber, the at least two thin-film electronic devices being provided by a plurality of thin-film layers formed on a substrate having opposing surfaces with the plurality of thin-film layers disposed adjacent the same one of the opposing surfaces, each of the at least two thin-film electronic devices being provided by a different subset of the plurality of thin-film layers,
- wherein operating includes operating a plurality of temperature control devices provided by the plurality of thin-film layers, with each temperature control device including a heater and a temperature sensor, to achieve different temperatures in distinct regions of the chamber at the same time.
A method of using a microfluidic device to analyze at least one nucleic acid in a sample, comprising:
- introducing a sample including a nucleic acid into a chamber;
- operating at least two thin-film electronic temperature control devices in the chamber, the at least two thin-film electronic devices being provided by a plurality of thin-film layers formed on a substrate having opposing surfaces with the plurality of thin-film layers disposed adjacent the same one of the opposing surfaces, each of the at least two thin-film electronic devices being provided by a different subset of the plurality of thin-film layers;
- and
- wherein operating includes operating a plurality of temperature control devices provided by the plurality of thin-film layers, with each temperature control device including a heater and a temperature sensor, to achieve different temperatures in distinct regions of the chamber at the same time,
- detecting the nucleic acid after operating the at least two thin-film electronic devices;
- wherein operating includes thermally cycling a region of the chamber in the presence of nucleic acid amplification reagents to effect nucleic acid amplification.
A method of using a microfluidic device to analyze at least one nucleic acid in a sample, comprising:
- introducing a sample including a nucleic acid into a chamber;
- operating at least two thin-film electronic temperature control devices in the chamber, the at least two thin-film electronic devices being provided by a plurality of thin-film layers formed on a substrate having opposing surfaces with the plurality of thin-film layers disposed adjacent the same one of the opposing surfaces, each of the at least two thin-film electronic devices being provided by a different subset of the plurality of thin-film layers;
- and
- wherein operating includes operating a plurality of temperature control devices provided by the plurality of thin-film layers, with each temperature control device including a heater and a temperature sensor, to achieve different temperatures in distinct regions of the chamber at the same time,
- detecting the nucleic acid after operating the at least two thin-film electronic devices;
- wherein operating includes moving the nucleic acid with respect to the chamber by generation of an electric field.
Dependencies are inferred from the claim text.