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US 7,741,121 B2Granted
System for purification and analysis of radiochemical products yielded by microfluidic synthesis devices
AssigneeSiemens Medical Solutions USA, Inc.
InventorsArkadij M Elizarov, Hartmuth C. Kolb, Jianzhong Zhang
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Abstract
The present application is generally directed to microfluidic devices and methods for the achievement and assessment of chemical and radiochemical purity of (microfluidic) radio-synthesis products. More particularly, the current application relates to systems for purification and analysis of radiochemical products yielded by microfluidic synthesis devices.
Claims42
A system for manipulating a radiolabeled compound-containing reaction mixture, the system comprising:
- i) a microfluidic analytical device comprising a microfluidic detection flow channel having an inlet and an outlet;
- ii) a first radiometric detector coupled to the top of the detection flow channel;
- and a second radiometric detectors coupled to the bottom of to the detection flow channel, for generating a signal corresponding to detection of the radiolabel in the detection flow channel, wherein said signal is registered simultaneously by both detectors, wherein the first and the second detectors are both operatively attached to a controller, the controller is configured so that any radiation signal not simultaneously received by both detectors is excluded from processing;
- iii) a spectrophotometric source for providing light to the detection flow channel;
- iv) a spectrophotometric detector for generating a spectrophotometric signal corresponding to detection of light from the detection flow channel to determine chemical identity and purity of the sample in the detection flow channel;
- and
- v) a high pressure liquid chromatography column onto which the reaction mixture can be loaded, the column having an inlet and an outlet, the inlet of the column being configured to receive the reaction mixture, and the outlet of the column being connected in fluid communication with the inlet of the detection flow channel of the device, wherein the column is selected such that the column can separate and purify the radiolabeled compound from the reaction mixture containing radiolabeled and non radiolabeled impurities;
- whereby when fluid containing the radiolabeled compound flows from the column into the detection flow channel, the radiometric detectors generate the signal and the spectrophotometric detector generates a spectrophotometric signal substantially simultaneously corresponding to the radiolabeled compound.
5 more elements
A method of manipulating a radiolabeled compound-containing reaction mixture, the method comprising:
- i) passing the reaction mixture through a system comprising
- a) a high pressure liquid chromatography column selected such that the column can separate and purify the radiolabeled compound from the reaction mixture containing radiolabeled and non radiolabeled impurities, the column having an inlet and an outlet, the inlet of the column being configured to receive the reaction mixture, and the outlet of the column being connected in fluid communication with the inlet of the detection flow channel of
- b) a microfluidic analytical device comprising a microfluidic detection flow channel having an inlet and an outlet;
- the device having coupled thereto
- 1) a first radiometric detector coupled to the top of the detector flow channel and a second radiometric detectors coupled to the bottom of the detection flow channel, for generating a signal corresponding to detection of the radiolabel in the detection flow channel, wherein said signal is registered simultaneously by both detectors;
- and wherein the first and the second detectors are both operatively attached to a controller, the controller is configured so that any radiation signal not simultaneously received by both detectors is excluded from processing;
- and
- wherein the first and second radiometric detectors are both operatively attached to a controller, and the controller is configured so that any radiation signal not simultaneously received by both detectors is excluded from processing;
- 2) a spectrophotometric source for providing light to the detection flow channel;
- and
- 3) a spectrophotometric detector for generating a spectrophotometric signal corresponding to detection of light from the detection flow;
- and
- ii) collecting a sample from the outlet of the detection flow channel when a spectrophotometric signal and the radiometric signal corresponding to the radiolabeled compound occur substantially simultaneously ,
- whereby the collected sample contains the radiolabeled compound.
11 more elements
Dependencies are inferred from the claim text.