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US 7,741,100 B2Granted
Method for highly expressing recombinant glucose dehydrogenase derived from filamentous fungi
AssigneeToyo Boseki Kabushiki Kaisha
InventorsMasao Kitabayashi, Yuji Tsuji, Hiroshi Kawaminami, Takahide Kishimoto, Yoshiaki Nishiya
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Abstract
The present invention provides a method for highly expressing a recombinant FAD-GDH protein derived from filamentous fungi, protein obtained by the method, and a regent for measuring glucose using the protein. According to the invention, the FAD-GDH can be highly expressed by altering DNA sequence coding for a signal peptide of FAD-GDH gene isolated from Aspergillus oryzae. FAD-GDH can be stably produced by adjusting pH of 7.1 to 7.3 during culture production.
Claims6
An isolated DNA sequence encoding the amino acid sequence of SEQ ID NO:
- 4, except that a part or all of a DNA sequence encoding a signal peptide sequence present at the N terminus of the amino acid sequence is substituted and/or deleted.
Dependencies are inferred from the claim text.