Back to results
US 7,741,098 B2Granted
Production of eukaryotic proteins and nucleic acid molecules in C. elegans
AssigneeneXyte AB
InventorsUwe H Sauer, Simon Tuck
Life of this patent
Estimating the term…
Loading patent…
Loading patent…
AssigneeneXyte AB
InventorsUwe H Sauer, Simon Tuck
Estimating the term…
Plasmid vectors for expression in Caenorhabditis elegans harbouring a heat inducible promoter nucleotide sequence, a synthetic intron nucleotide sequence optionally containing a Shine-Dalgarno sequence for efficient shuttling between C. elegans and E. coli, optionally a nucleotide sequence coding for a nuclear localisation signal or secretion signal, a nucleotide sequence coding for a recognizable tag, optionally a nucleotide sequence coding for a fluorescent protein, a nucleotide sequence coding for a protease cleavage site, a multiple cloning site containing a nucleotide sequence coding for an eukaryotic, such as human, protein or a nucleic acid molecule and a nucleotide sequence coding for termination of translation, are described. Methods of particularly large scale production of eukaryotic, such as human, proteins and nucleic acid molecules in nematodes are also described.
Plasmid vector for expression in Caenorhabditis elegans and in Escherichia coli comprising in the 5′ to 3′ direction of transcription operably linked to each other a heat shock promoter nucleotide sequence, a synthetic intron nucleotide sequence containing a Shine-Dalgarno sequence, optionally a nucleotide sequence coding for a nuclear localization signal or a secretion signal, a nucleotide sequence coding for a recognizable tag, optionally a nucleotide sequence coding for a fluorescent protein, a nucleotide sequence coding for a protease cleavage site, a multiple cloning site containing a nucleic acid molecule or a nucleotide sequence coding for a eukaryotic protein, and a nucleotide sequence coding for termination of translation
Plasmid vector for expression in Caenorhabditis elegans and in Escherichia coli comprising in the 5′ to 3′ direction of transcription operably linked to each other a heat shock promoter nucleotide sequence, a synthetic intron nucleotide sequence containing a Shine-Dalgarno sequence, optionally a nucleotide sequence coding for a nuclear localization signal or a secretion signal, a nucleotide sequence coding for a recognizable tag, optionally a nucleotide sequence coding for a fluorescent protein, a nucleotide sequence coding for a protease cleavage site, a multiple cloning site containing a nucleic acid molecule or a nucleotide sequence coding for a eukaryotic protein, and a nucleotide sequence coding for termination of translation
Plasmid vector for expression in Caenorhabditis elegans and in Escherichia coli comprising in the 5′ to 3′ direction of transcription operably linked to each other a heat shock promoter nucleotide sequence, a synthetic intron nucleotide sequence containing a Shine-Dalgamo sequence, a multiple cloning site containing a nucleic acid molecule or a nucleotide sequence coding for a eukaryotic protein, a nucleotide sequence coding for a protease cleavage site, optionally a nucleotide sequence coding for a fluorescent protein, optionally a nucleotide sequence coding for a nuclear localization signal or a secretion signal, a nucleotide sequence coding for a recognizable tag and a nucleotide sequence coding for termination of translation
Dependencies are inferred from the claim text.