Glucose dehydrogenase and process for producing the dehydrogenase
OwnerKoji Sodeinventor
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Abstract
A novel glucose dehydrogenase, which is an enzyme that has high substrate specificity, can be produced at a low cost, is not affected by oxygen dissolved in a measurement sample and, in particular, has superior thermal stability is obtained by culturing a microorganism belonging to the genus Burkhorderia and having glucose dehydrogenase producing ability in a medium and collecting glucose dehydrogenase from the medium and/or cells of the microorganism.
Claims4
A method for producing glucose dehydrogenase comprising the steps of culturing Burkholderia cepacia having glucose dehydrogenase producing ability in a medium, and collecting glucose dehydrogenase from the medium and/or cells of Burkholderia cepacia, wherein the glucose dehydrogenase has the following properties:
- (i) the enzyme has an action of catalyzing dehydrogenation reaction of glucose;
- (ii) the enzyme consists of α subunit(s) having a molecular weight of about 60 kDa and β subunit(s) which is cytochrome C having a molecular weight of about 43 kDa in SDS-polyacrylamide gel electrophoresis under a reducing condition;
- (iii) the enzyme shows a molecular weight of about 380 kDa in gel filtration chromatography using TSK Gel G3000SW;
- and
- (iv) the enzyme shows an optimal reaction temperature around 45° C.
Dependencies are inferred from the claim text.