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AssigneeVerenium Corporation
InventorsTim Hitchman, Dan E. Robertson, Masao Hiraiwa, Yoko Phillips, Kevin A. Gray
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The invention provides laccases, polynucleotides encoding these enzymes, the use of such polynucleotides and polypeptides. In one aspect, the invention relates to the enzymatic production of nootkatone by way of the conversion of valencene using proteins having a laccase activity, e.g., a novel laccase of the invention. In one aspect, the invention provides methods of depolymerizing lignin, e.g., in a pulp or paper manufacturing process, using a polypeptide of the invention. In another aspect, the invention provides methods for oxidizing products that can be mediators of laccase-catalyzed oxidation reactions, e.g., 2,2-azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS), 1-hydroxybenzotriazole (HBT), 2,2,6,6-tetramethylpiperidin-1-yloxy (TEMPO), dimethoxyphenol, and the like.
An isolated, synthetic, or recombinant nucleic acid comprising:
An isolated, synthetic, or recombinant nucleic acid comprising a nucleic acid sequence having at least 97% sequence identity to SEQ ID NO.:23, wherein the nucleic acid encodes a polypeptide having a laccase activity.
An isolated, synthetic, or recombinant nucleic acid comprising a nucleic acid sequence having at least 99% sequence identity to SEQ ID No.:23, wherein the nucleic acid encodes a polypeptide having a laccase activity.
An isolated, synthetic, or recombinant nucleic acid comprising the sequence of SEQ ID NO:23.
The isolated, synthetic, or recombinant nucleic acid as in any one of the preceding claims, wherein the nucleic acid comprises a sequence that encodes a polypeptide having laccase activity and having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:24.
Dependencies are inferred from the claim text.