Lipid remodeling of GPI-anchored proteins
AssigneeNational Institute of Advanced Industrial Science and Technology
InventorsYoshifumi Jigami, Takehiko Yoko-O, Mariko Umemura, Morihisa Fujita
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Abstract
This invention provides proteins and genes thereof involved in the GPI lipid remodeling process and, thereby and constructing a system for screening for useful substances such as anticancer agents and a system for detecting abnormalities in the GPI lipid remodeling process.
Claims3
A method for converting the phosphatidylinositol structure of a GPI-anchored protein to a lysophosphatidylinositol structure, comprising incubating said GPI-anchored protein in the phosphatidylinositol form in the presence of an enzyme, wherein:
- (i) said enzyme shares 92% or more homology with the amino acid sequence of SEQ ID NO:
- 2 or SEQ ID NO:
- 4;
- and
- (ii) said enzyme has the activity of converting the phosphatidylinositol form of a GPI-anchored protein to the lysophosphatidylinositol form.
A method for screening for a substance that promotes or inhibits the activity of an enzyme, comprising:
- (i) incubating a GPI-anchored protein in the phosphatidyliniositol form and said enzyme in the absence or presence of said substance;
- and
- (ii) detecting any change in the amount of the lysophosphatidylinositol form of said GPI-anchored protein,
- wherein:
- (a) said enzyme shares 92% or more homology with the amino acid sequence of SEQ ID NO:
- 2 or SEQ ID NO:
- 4;
- and
- (b) said enzyme has the activity of converting the phosphatidylinositol form of a GPI-anchored protein to the lysophosphatidylinositol form.
Dependencies are inferred from the claim text.