Process for producing L-arginine, L-ornithine or L-citrulline
AssigneeKyowa Hakko Bio Co., Ltd.
InventorsMasato Ikeda, Tetsuo Nakano, Satoshi Mitsuhashi, Mikiro Hayashi, Kenji Tanaka
Life of this patent
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Abstract
The present invention provides a polypeptide which has: (i) an amino acid sequence wherein one or more amino acid residues are substituted in the region at positions 20 to 38 from the N terminus of the amino acid sequence shown in SEQ ID NO: 1; or (ii) an amino acid sequence wherein one or more amino acid residues are substituted in the region at positions 20 to 38 from the N terminus of the amino acid sequence shown in SEQ ID NO: 1 and one or more amino acid residues are deleted, substituted or added in the region at positions 1 to 19 or 39 to 294; and which has N-acetylglutamate kinase activity.
Claims10
A process for producing L-arginine, L-ornithine or L-citrulline, comprising the steps of:
- culturing in a medium an isolated microorganism carrying a DNA comprising a nucleotide sequence selected from the group consisting of SEQ ID NOS:
- 4, 6, 8, 10 and 12;
- allowing L-arginine, L-ornithine or L-citrulline to form and accumulate in the medium;
- and
- recovering L-arginine, L-ornithine or L-citrulline from the medium.
A process for producing L-arginine, L-ornithine or L-citrulline, comprising the steps of:
- culturing in a medium an isolated microorganism carrying a DNA which hybridizes with DNA consisting of a nucleotide sequence complementary to the nucleotide sequence of DNA encoding SEQ ID NO:
- 1 at 65° C. in 0.7-1.0 mol/l sodium chloride followed by washing at 65° C. with 0.1-2 ×SSC solution, said DNA being selected from the group consisting of the following (i) to (iii):
- (i) DNA encoding a polypeptide wherein the amino acid residue corresponding to the residue at position 26 from the N terminus of the amino acid sequence shown in SEQ ID NO:
- 1 is an amino acid residue other than L-alanine;
- (ii) DNA encoding a polypeptide wherein the amino acid residue corresponding to the residue at position 31 from the N terminus of the amino acid sequence shown in SEQ ID NO:
- 1 is an amino acid residue other than L-methionine;
- and
- (iii) DNA encoding a polypeptide wherein the amino acid residue corresponding to the residue at position 26 from the N terminus of the amino acid sequence shown in SEQ ID NO:
- 1 is an amino acid residue other than L-alanine and the amino acid residue corresponding to the residue at position 31 is an amino acid residue other than L-methionine, and
- which encodes a polypeptide having N-acetylglutamate kinase activity;
- allowing L-arginine, L-ornithine or L-citrulline to form and accumulate in the medium;
- and
- recovering L-arginine, L-ornithine or L-citrulline from the medium.
A process for producing L-arginine, L-ornithine or L-citrulline, comprising the steps of:
- culturing in a medium an isolated microorganism carrying a DNA which hybridizes with DNA consisting of a nucleotide sequence complementary to the nucleotide sequence of DNA encoding SEQ ID NO:
- 1 at 65° C. in 0.7-1.0 mol/l sodium chloride followed by washing at 65° C with 0.1-2 ×SSC solution, selected from the group consisting of the following (i) to (iii):
- (i) DNA having a nucleotide sequence wherein the region corresponding to the region at positions 76 to 78 from the 5′ end of the nucleotide sequence shown in SEQ ID NO:
- 2 is guanine-thymidine-thymidine, cytosine-thymidine-guanine or adenine-thymidine-cytosine;
- (ii) DNA having a nucleotide sequence wherein the region corresponding to the region at positions 91 to 93 from the 5′ end of the nucleotide sequence shown in SEQ ID NO:
- 2 is guanine-thymidine-guanine;
- and
- (iii) DNA having a nucleotide sequence wherein the region corresponding to the region at positions 76 to 78 from the 5′ end of the nucleotide sequence shown in SEQ ID NO:
- 2 is guanine-thymidine-thymidine, cytosine-thymidine-guanine or adenine-thymidine-cytosine, and the region corresponding to the region at positions 91 to 93 is guanine-thymidine-guanine;
- and
- which encodes a polypeptide having N-acetylglutamate kinase activity;
- allowing L-arginine, L-ornithine or L-citrulline to form and accumulate in the medium;
- and
- recovering L-arginine, L-ornithine or L-citrulline from the medium.
Dependencies are inferred from the claim text.