Method for the generation of genetically modified vertebrate precursor lymphocytes and use thereof for the production of heterologous binding proteins
Assignee4-Antibody AG
InventorsUlf Grawunder, Georg Friedrich Melchers
Life of this patent
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Abstract
The present invention generally relates to the fields of genetic engineering and antibody production. In particular, it relates to the generation of genetically modified vertebrate precursor lymphocytes that have the potential to differentiate into more mature lymphoid lineage cells, and to the use thereof for the production of any heterologous antibody or binding protein.
Claims25
A method for the generation of vertebrate precursor B lymphocytes that can be used for the production of an antibody or an antigen-binding fragment(s) thereof, comprising:
- (a) genetically modifying isolated vertebrate precursor B lymphocytes, which
- (i) are derived from primary lymphoid organs, and
- (ii) have the potential to differentiate into mature B lymphoid lineage cells, by introducing at least one exogenous genetic element encoding at least one antibody or antigen-binding fragment(s) thereof;
- and
- (b) effecting differentiation of said genetically modified precursor B lymphocytes into mature lymphoid lineage cells either in vitro or in vivo, thereby generating B lymphocytes capable of producing an antibody or an antigen-binding fragment(s) thereof.
A method for the production of an antibody, antibodies or (an) antigen-binding fragment(s) thereof, comprising:
- (a) genetically modifying vertebrate precursor B lymphocytes, which
- (i) are isolated from primary lymphoid organs, and
- (ii) have the potential to differentiate into mature B lymphoid lineage cells, by introducing at least one exogenous genetic element encoding at least one antibody or antigen-binding fragment(s) thereof;
- or, alternatively, isolating genetically modified vertebrate precursor B lymphocytes from primary lymphoid organs, wherein said genetically modified vertebrate precursor B lymphocytes have the potential to differentiate into mature B lymphoid lineage cells, and carry at least one exogenous genetic element encoding at least one antibody or antigen-binding fragment thereof;
- (b) effecting differentiation of said genetically modified precursor B lymphocytes into mature lymphoid lineage cells either in vitro or in vivo, thereby generating genetically modified and differentiated vertebrate B lymphocytes capable of producing an antibody or (an) antigen-binding fragment(s) thereof;
- (c) effecting expression of the antibodies, antibody, or antigen-binding fragment(s) thereof.
Dependencies are inferred from the claim text.