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US 7,741,073 B2Granted
Plastidial targeting peptide
AssigneeGenoplante-Valor
InventorsStephane Miras, Daniel Salvi, Norbert Rolland, Jacques Joyard, Myriam Ferro, Jerome Garin, Didier Grunwald
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Abstract
The invention relates to a non-cleavable, plastidial targeting polypeptide derived from a protein from the inner membrane of plant chloroplasts. Said peptide is particularly suitable for importing proteins of interest in plants.
Claims17
An isolated polynucleotide encoding an intraplastid-targeting polypeptide, wherein the polypeptide consists of:
- domain A consisting of:
- i) amino acids 60-100 of SEQ ID NO:
- 1;
- or ii) a fragment of a protein of an inner membrane of a chloroplast envelope, said protein being recognized by an antibody directed against SEQ ID NO:
- 1, and said fragment having at least 70% identity with amino acids 60-100 of SEQ ID NO:
- 1 or SEQ ID NO:
- 3 and when combined with domain B has plastid targeting activity;
- and
- a domain B located at the N-terminal end of domain A consisting of iii) a fragment of amino acids 1-59 of SEQ ID NO:
- 1, said fragment including at least amino acids 49-59 of SEQ ID NO:
- 1 or SEQ ID NO:
- 3, or iv) a fragment of a protein of an inner membrane of a chloroplast envelope, said protein being recognized by antibodies directed against the polypeptide SEQ ID NO:
- 1, and said fragment having at least 60% identity with said fragment iii) and when combined with domain A has plastid targeting activity.
10 more elements
Dependencies are inferred from the claim text.