Polypeptide variants
AssigneeGenentech, Inc.
InventorsEsohe Ekinaduese Idusogie, Leonard G. Presta, Michael George Mulkerrin
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Abstract
A variant of a polypeptide comprising a human IgG Fc region is described, which variant comprises an amino acid substitution at one or more of amino acid positions 270, 322, 326, 327, 329, 331, 333 or 334 of the human IgG Fc region. Such variants display altered effector function. For example, C1q binding and/or complement dependent cytotoxicity (CDC) activity may be altered in the variant polypeptide. The application also discloses a variant of a parent polypeptide comprising a human IgG Fc region, which variant has a better binding affinity for human C1q than the parent polypeptide.
Claims20
A process of producing a polypeptide variant comprising culturing a host cell comprising an isolated nucleic acid encoding a variant of a parent antibody or immunoadhesin polypeptide comprising a human IgG Fc region, which variant comprises an amino acid substitution at amino acid position 326, 327, 333 334, or combination thereof of the human IgG Fc region, where the numbering of the residues in the IgG Fc region is that of the EU index as in Kabat.
Dependencies are inferred from the claim text.