Luciferase-based assays
AssigneePromega Corporation
InventorsErika Hawkins, James J. Cali, Samuel Kin Sang Ho, Martha A. O'Brien, Richard Somberg, Robert F. Bulleit, Keith V. Wood
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Abstract
A method and kit is provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent.
Claims8
A method for protecting activity of a luciferase enzyme from an interfering effect of a test compound in a cell free test compound screening environment, said method comprising steps of:
- contacting the luciferase enzyme with a detergent to form a first composition;
- and
- contacting the first composition with a second composition, said second composition comprising the test compound,
- wherein the detergent is present in an amount sufficient to substantially protect the activity of the luciferase enzyme and reduce the likelihood of a false positive resulting from the interfering effect of the test compound, relative to the absence of detergent, in a test compound screening environment.
Dependencies are inferred from the claim text.