Toxoplasma gondii oocyst protein
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsDolores Hill, Dante S Zarlenga, Cathleen Coss, Jitender P Dubey
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Abstract
Recombinant proteins have been developed for the detection of Toxoplasma gondii oocyst proteins for example in biological fluids. Isolated DNA sequences which encode these proteins have also been developed. The DNA sequences may be inserted into recombinant DNA molecules such as cloning vectors or expression vectors for the transformation of cells and the production of the proteins.
Claims9
An isolated protein consisting of the amino acid sequence of SEQ ID NO:
- 1.
A fusion protein consisting of the amino acid sequence shown in SEQ ID NO:1 and wherein said protein is antigenic and effective to elicit an immune response against Toxoplasma gondii oocysts in a host animal, wherein said protein is operably coupled to another unrelated polypeptide sequence.
A method of identifying Toxoplasma gondii oocysts antibodies in a subject, said method comprising contacting a test sample suspected of containing antibodies to Toxoplasma gondii oocysts with an antigen wherein the antigen is a protein consisting of the amino acid sequence set forth in SEQ ID NO:
- 1 and detecting antibody-antigen binding, wherein binding indicates the presence of Toxoplasma gondii oocysts antibodies;
- wherein said test sample is obtained from said subject.
Dependencies are inferred from the claim text.