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US 7,741,059 B2Granted
Methods and compositions for detection of Ehrlichia chaffeensis (p120)
AssigneeIDEXX Laboratories, Inc.
InventorsThomas Patrick O'Connor, Jr., Jill M. Saucier
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Abstract
The invention provides methods and compositions for the detection of Ehrlichia chaffeensis.
Claims14
A method of detecting antibodies or antigen-binding antibody fragments that specifically bind an Ehrlichia chaffeensis polypeptide in a test sample, comprising:
- (a) contacting a purified polypeptide comprising SEQ ID NO:1, 2, 4, or 5 with the test sample, under conditions that allow polypeptide/antibody complexes to form;
- and wherein the purified polypeptide consists of less than about 50 contiguous naturally occurring Ehrlichia chaffeensis amino acids, and wherein the purified polypeptide does not specifically bind Ehrlichia canis antibodies in the test sample;
- (b) detecting the polypeptide/antibody complexes;
- wherein the detection of the polypeptide/antibody complexes is an indication that antibodies specific for Ehrlichia chaffeensis are present in the test sample.
1 more element
A method of detecting an Ehrlichia chaffeensis infection in a subject comprising:
- (a) obtaining a biological sample from the subject;
- (b) contacting a purified polypeptide comprising SEQ ID NO:1, 2, 4 or 5 with the biological sample under conditions that allow polypeptide/antibody complexes to form;
- wherein, the purified polypeptide consists of less than about 50 contiguous naturally occurring Ehrlichia chaffeensis amino acids;
- (c) detecting the polypeptide/antibody complexes;
- wherein the detection of the polypeptide/antibody complexes is an indication that the subject has an Ehrlichia chaffeensis infection and wherein the method does not detect Ehrlichia canis infection in the subject.
2 more elements
Dependencies are inferred from the claim text.