Processes for purification of recombinant proteins
AssigneeSigma-Aldrich Co.
InventorsRichard J. Mehigh, Eliezer Kopf, Efrat Reem, Edward B. Watson, III
Life of this patent
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Abstract
Processes for the detection and purification of peptides and proteins comprising a metal ion-affinity peptide having a high affinity for coordinated metals and also being highly antigenic are described. Antibodies for use in the processes are also described.
Claims29
An antibody that binds to a metal ion-affinity peptide in a polypeptide, protein or protein fragment represented by the formula R1-Sp1-(His-Z1-His-Arg-His-Z2-His)-Sp2-R2, wherein (His-Z1-His-Arg-His-Z2-His) is the metal ion-affinity peptide, R1 is hydrogen, a polypeptide, protein or protein fragment;
- Sp1 is a covalent bond or a spacer comprising at least one amino acid residue;
- R2 is hydrogen, a polypeptide, protein or protein fragment;
- Sp2 is a covalent bond or a spacer comprising at least one amino acid residue;
- Z1 is an amino acid residue selected from the group consisting of Ala, Arg, Asn, Asp, Gln, Glu, Ile, Lys, Phe, Pro, Ser, Thr, Trp, and Val;
- and Z2 is an amino acid residue selected from the group consisting of Ala, Arg, Asn, Asp, Cys, Gin, Glu, Gly, Ile, Leu, Lys, Met, Pro, Ser, Thr, Tyr, and Val.
An antibody that binds to a metal ion-affinity peptide in a polypeptide, protein or protein fragment represented by the formula R1-Sp1-(His-Z1-His-Arg-His-Z2-His)t-Sp2-R2, wherein (His-Z1-His-Arg-His-Z2-His) is the metal ion-affinity peptide, t is at least 2, R1 is hydrogen, a polypeptide, protein or protein fragment, Sp1 is a covalent bond or a spacer comprising at least one amino acid residue, R2 is hydrogen, a polypeptide, protein or protein fragment, Sp2 is a covalent bond or a spacer comprising at least one amino acid residue, Z1 is an amino acid residue selected from the group consisting of Ala, Arg, Asn, Asp, Gln, Glu, Ile, Lys, Phe, Pro, Ser, Thr, Trp, and Val, and Z2 is an amino acid residue selected from the group consisting of Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, Ile, Leu, Lys, Met, Pro, Ser, Thr, Tyr, and Val.
An antibody that binds to a metal ion-affinity peptide in a polypeptide, protein or protein fragment represented by the formula R1-Sp1-[(His-Z1-His-Arg-His-Z2-His)-Sp2]t-R2, wherein (His-Z1-His-Arg-His-Z2-His) is the metal ion-affinity peptide, t is at least 2, R1 is hydrogen, a polypeptide, protein or protein fragment, Sp1 is a covalent bond or a spacer comprising at least one amino acid residue, R2 is hydrogen, a polypeptide, protein or protein fragment, Sp2 is a covalent bond or a spacer comprising at least one amino acid residue, Z1 is an amino acid residue selected from the group consisting of Ala, Arg, Asn, Asp, Gln, Glu, Ile, Lys, Phe, Pro, Ser, Thr, Trp, and Val, and Z2 is an amino acid residue selected from the group consisting of Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, Ile, Leu, Lys, Met, Pro, Ser, Thr, Tyr, and Val;
- and each Sp2 of the recombinant polypeptides, proteins or protein fragments may be the same or different.
Dependencies are inferred from the claim text.